Ethanol from Oil Palm Empty Fruit Bunch via Dilute-Acid Hydrolysis and Fermentation by Mycur Indicus and saccharomyces cerevisiae
Journal article, 2011
Oil Palm Empty Fruit Bunch (OPEFB) was hydrolyzed in a one-stage hydrolysis using dilute-sulfuric acid (0.2, 0.8%) at 170-230°C with a holding time of 5 and 15 min. The maximum yield of xylose was 135.94 g kg-1 OPEFB, obtained at 0.8% acid, 190°C and 5 min. The maximum yield of glucose was 62.70 g kg-1 OPEFB, obtained at 0.8% acid, 210°C and 5 min. Based on these results, two-stage hydrolysis was performed to produce hydrolyzates for the fermentation process. Hydrolyzate from the first stage was fermented by Mucor indicus while the hydrolyzate from the second stage was fermented by Saccharomyces cerevisiae. The corresponding ethanol yields were 0.45 and 0.46 g ethanol g-1 sugar consumed.