Industrial glucoamylase fed-batch benefits from oxygen limitation and high osmolarity
Journal article, 2012

The market for glucoamylase is large and very competitive and the production process has been optimized through several decades. So far a thorough characterization of the process has not been published, but previous academic reports suggest that the process suffers from severe byproduct formation. In this study we have carried out a thorough characterization of a process as close as possible to the industrial reality. The results show that the oxygen-limited phases of the process have the highest glucoamylase yields on carbon and that the byproducts are efficiently reused in late phases of the process. An alternative process with low glucose concentration show that high osmolarity is beneficial for the process, and we conclude that oxygen limitation, high osmolarity, and the associated byproduct metabolism are important for the efficiency of the process.

Aspergillus niger


enzyme production


oxygen limitation


L. Pedersen

Technical University of Denmark (DTU)

K. Hansen

Novozymes A/S

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

A. E. Lantz

Technical University of Denmark (DTU)

J. Thykaer

Technical University of Denmark (DTU)

Biotechnology and Bioengineering

0006-3592 (ISSN) 1097-0290 (eISSN)

Vol. 109 1 116-124

Driving Forces

Sustainable development

Subject Categories

Industrial Biotechnology

Areas of Advance


Life Science Engineering (2010-2018)



More information

Latest update

7/3/2018 1