Mechanistic Insight into the Structure and Dynamics of Entangled and Hydrated lambda-Phage DNA
Journal article, 2012

Intrinsic dynamics of DNA plays a crucial role in DNA protein interactions and has been emphasized as a possible key component for in vivo chromatin organization. We have prepared an entangled DNA microtube above the overlap concentration by exploiting the complementary cohesive ends of lambda-phage DNA, which is confirmed by atomic force microscopy and agarose gel electrophoresis. Photon correlation spectroscopy further confirmed that the entangled solutions are found to exhibit the classical hydrodynamics of a single chain segment on length scales smaller than the hydrodynamic length scale of single lambda-phage DNA molecule. We also observed that in 41.6% (gm water/gm DNA) hydrated state, lambda-phage DNA exhibits a dynamic transition temperature (T-dt) at 187 K and a crossover temperature (T-c) at 246 K. Computational insight reveals that the observed structure and dynamics of entangled lambda-phage DNA are distinctively different from the behavior of the corresponding unentangled DNA with open cohesive ends, which is reminiscent with our experimental observation.





circular dna

atomic-force microscopy


web server


anomalous diffusion


S. Chakraborty

Saroj Mohan Institute of Technology

Takashi Uematsu

Chalmers, Applied Physics, Condensed Matter Physics

Christer Svanberg

Chalmers, Applied Physics, Condensed Matter Physics

Per Jacobsson

Chalmers, Applied Physics, Condensed Matter Physics

Jan Swenson

Chalmers, Applied Physics, Condensed Matter Physics

Michael Zäch

Chalmers, Applied Physics, Chemical Physics

R. Trehan

Tougaloo College

G. Armstrong

Tougaloo College

Bidisa Sengupta

Tougaloo College

Journal of Physical Chemistry A

1089-5639 (ISSN) 1520-5215 (eISSN)

Vol. 116 17 4274-4284

Subject Categories

Physical Sciences

Chemical Sciences

Areas of Advance

Life Science Engineering (2010-2018)

Materials Science



More information