Mechanistic Insight into the Structure and Dynamics of Entangled and Hydrated lambda-Phage DNA
Artikel i vetenskaplig tidskrift, 2012

Intrinsic dynamics of DNA plays a crucial role in DNA protein interactions and has been emphasized as a possible key component for in vivo chromatin organization. We have prepared an entangled DNA microtube above the overlap concentration by exploiting the complementary cohesive ends of lambda-phage DNA, which is confirmed by atomic force microscopy and agarose gel electrophoresis. Photon correlation spectroscopy further confirmed that the entangled solutions are found to exhibit the classical hydrodynamics of a single chain segment on length scales smaller than the hydrodynamic length scale of single lambda-phage DNA molecule. We also observed that in 41.6% (gm water/gm DNA) hydrated state, lambda-phage DNA exhibits a dynamic transition temperature (T-dt) at 187 K and a crossover temperature (T-c) at 246 K. Computational insight reveals that the observed structure and dynamics of entangled lambda-phage DNA are distinctively different from the behavior of the corresponding unentangled DNA with open cohesive ends, which is reminiscent with our experimental observation.

light-scattering

molecules

generation

transition

circular dna

atomic-force microscopy

water

web server

aqueous-solutions

anomalous diffusion

Författare

S. Chakraborty

Saroj Mohan Institute of Technology

Takashi Uematsu

Chalmers, Teknisk fysik, Kondenserade materiens fysik

Christer Svanberg

Chalmers, Teknisk fysik, Kondenserade materiens fysik

Per Jacobsson

Chalmers, Teknisk fysik, Kondenserade materiens fysik

Jan Swenson

Chalmers, Teknisk fysik, Kondenserade materiens fysik

Michael Zäch

Chalmers, Teknisk fysik, Kemisk fysik

R. Trehan

Tougaloo College

G. Armstrong

Tougaloo College

Bidisa Sengupta

Tougaloo College

Journal of Physical Chemistry A

1089-5639 (ISSN) 1520-5215 (eISSN)

Vol. 116 4274-4284

Ämneskategorier

Fysik

Kemi

Styrkeområden

Livsvetenskaper och teknik

Materialvetenskap

DOI

10.1021/jp2108363