Non-linear microscopy of smooth muscle cells in artificial extracellular matrices made of cellulose
Journal article, 2012
Non-linear microscopy has been used to characterize bovine smooth muscle cells and their proliferation, migration, and differentiation in hydrogel cellulose scaffolds, toward the development of fully functional blood vessel implants. The extracellular matrix (ECM) composed of cellulose and endogenous collagen fibers was imaged using Second Harmonic Generation (SHG) microscopy and the cell morphology by Coherent Anti-Stokes Raman Scattering (CARS) microscopy. Images prove that cells adhere on the cellulose scaffold without additional surface modification and that both contractile and proliferating phenotypes are developed. This work shows that non-linear microscopy contributes with unique insights in cell interactions with (artificial) ECM components and has the potential to become an established characterization method in tissue engineering.
bacterial cellulose
dynamics
non-linear microscopy
tissue engineering
microbial cellulose
culture
adhesions
smooth muscle cells
phenotype
migration
scaffolds
substrate
CARS
collagen
SHG
Author
Christian Brackmann
Chalmers, Chemical and Biological Engineering, Molecular Imaging
Jan-Olof Dahlberg
Chalmers, Chemical and Biological Engineering, Molecular Imaging
N. E. Vrana
Dublin City University
C. Lally
Dublin City University
Paul Gatenholm
Chalmers, Chemical and Biological Engineering, Polymer Technology
Annika Enejder
Chalmers, Chemical and Biological Engineering, Molecular Imaging
Journal of Biophotonics
1864-063X (ISSN) 18640648 (eISSN)
Vol. 5 5-6 404-414Areas of Advance
Life Science Engineering (2010-2018)
Subject Categories
Chemical Sciences
DOI
10.1002/jbio.201100141