Non-linear microscopy of smooth muscle cells in artificial extracellular matrices made of cellulose
Artikel i vetenskaplig tidskrift, 2012

Non-linear microscopy has been used to characterize bovine smooth muscle cells and their proliferation, migration, and differentiation in hydrogel cellulose scaffolds, toward the development of fully functional blood vessel implants. The extracellular matrix (ECM) composed of cellulose and endogenous collagen fibers was imaged using Second Harmonic Generation (SHG) microscopy and the cell morphology by Coherent Anti-Stokes Raman Scattering (CARS) microscopy. Images prove that cells adhere on the cellulose scaffold without additional surface modification and that both contractile and proliferating phenotypes are developed. This work shows that non-linear microscopy contributes with unique insights in cell interactions with (artificial) ECM components and has the potential to become an established characterization method in tissue engineering.

bacterial cellulose

dynamics

non-linear microscopy

tissue engineering

microbial cellulose

culture

adhesions

smooth muscle cells

phenotype

migration

scaffolds

substrate

CARS

collagen

SHG

Författare

Christian Brackmann

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

Jan-Olof Dahlberg

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

N. E. Vrana

Dublin City University

C. Lally

Dublin City University

Paul Gatenholm

Chalmers, Kemi- och bioteknik, Polymerteknologi

Annika Enejder

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

Journal of Biophotonics

1864-063X (ISSN)

Vol. 5 5-6 404-414

Styrkeområden

Livsvetenskaper och teknik

Ämneskategorier

Kemi

DOI

10.1002/jbio.201100141