Chemical images of marine bio-active compounds by surface enhanced Raman spectroscopy and transposed orthogonal partial least squares (T-OPLS)
Journal article, 2012
Surface enhanced Raman spectroscopy combined with transposed Orthogonal Partial Least Squares (T-OPLS) was shown to produce chemical images of the natural antibacterial surface-active compound 1,1,3,3-tetrabromo-2-heptanone (TBH) on Bonnemaisonia hamifera. The use of gold colloids function-alised with the internal standard 4-mercapto-benzonitrile (MBN) made it possible to create images of the relative concentration of TBH over the surfaces. A gradient of TBH could be mapped over and in the close vicinity of the B. hamifera algal vesicles at the attomol/pixel level. T-OPLS produced a measure of the spectral correlation for each pixel of the hyperspectral images whilst not including spectral variation that was linearly independent of the target spectrum. In this paper we show the possibility to retrieve specific spectral information with a low magnitude in a complex matrix.
pls
1
Surface enhanced Raman spectroscopy
fluorescence
red alga-bonnemaisonia
Bonnemaisonia hamifera 1
scattering
information
3
3-Tetrabromo-2-heptanone
chemometrics
bacterial-colonization
hamifera
feasibility
Author
Aamer Abbas
University of Gothenburg
Mats Josefson
AstraZeneca AB
Göran M. Nylund
University of Gothenburg
Henrik Pavia
University of Gothenburg
Katarina Abrahamsson
University of Gothenburg
Analytica Chimica Acta
0003-2670 (ISSN) 1873-4324 (eISSN)
Vol. 737 37-44Subject Categories (SSIF 2011)
Biochemistry and Molecular Biology
Ecology
DOI
10.1016/j.aca.2012.05.047