Chemical images of marine bio-active compounds by surface enhanced Raman spectroscopy and transposed orthogonal partial least squares (T-OPLS)
Artikel i vetenskaplig tidskrift, 2012

Surface enhanced Raman spectroscopy combined with transposed Orthogonal Partial Least Squares (T-OPLS) was shown to produce chemical images of the natural antibacterial surface-active compound 1,1,3,3-tetrabromo-2-heptanone (TBH) on Bonnemaisonia hamifera. The use of gold colloids function-alised with the internal standard 4-mercapto-benzonitrile (MBN) made it possible to create images of the relative concentration of TBH over the surfaces. A gradient of TBH could be mapped over and in the close vicinity of the B. hamifera algal vesicles at the attomol/pixel level. T-OPLS produced a measure of the spectral correlation for each pixel of the hyperspectral images whilst not including spectral variation that was linearly independent of the target spectrum. In this paper we show the possibility to retrieve specific spectral information with a low magnitude in a complex matrix.

feasibility

Surface enhanced Raman spectroscopy

hamifera

3

1

red alga-bonnemaisonia

scattering

chemometrics

Bonnemaisonia hamifera 1

pls

bacterial-colonization

information

fluorescence

3-Tetrabromo-2-heptanone

Författare

Aamer Abbas

Chalmers, Kemi- och bioteknik

Mats Josefson

Göran M. Nylund

Göteborgs universitet

Henrik Pavia

Göteborgs universitet

Katarina Abrahamsson

Chalmers, Kemi- och bioteknik

Analytica Chimica Acta

0003-2670 (ISSN)

Vol. 737 37-44

Ämneskategorier

Biokemi och molekylärbiologi

Ekologi

DOI

10.1016/j.aca.2012.05.047