Controlling and Monitoring Orientation of DNA Nanoconstructs on Lipid Surfaces

Erik Lundberg; Bobo Feng; Amir Saeid Mohammadi; Marcus Wilhelmsson; Bengt Nordén

doi:10.1021/la304178f

Controlling and Monitoring Orientation of DNA Nanoconstructs on Lipid Surfaces
Journal article, 2013

Its extraordinary self-assembly property, with potential to form nonperiodic structures with unique addressability, makes DNA ideal for fabrication of advanced nanostructures. We here demonstrate the controllable tethering of a hexagonal DNA nanostructure in two distinct orientations at the lipid bilayer of a liposome functioning as a soft-matter support. With polarized light (linear dichroism) applied to the flow-aligned liposomes, we show that the construct is preferentially in a parallel alignment with the lipid surface when two anchors are attached while with one anchor only a perpendicular orientation is observed.

vesicle fusion

nucleic-acids

finite-size

linear dichroism

energy-transfer

wires

nanostructures

reduced dichroism curves

membrane

origami

photonic