Human fibroblast and human bone marrow cell response to lithographically nanopatterned adhesive domains on protein rejecting substrates.
Journal article, 2007
The separate influence of topographical and chemical cues on cell attachment and spreading are well documented; however, that of duel-cue substrates is less so. In this study graft copolymers that sterically stabilize biological surfaces were employed alongside nanotopographical features fabricated by colloidal lithography. This resulted in the production of a range of substrates whereby the effect of chemistry and or topography on both on human fibroblast and bone marrow cell adhesion and spreading could be observed. The current studies indicate an enhancement of cell response as a consequence of modifications in material topography, whereas the current selected chemical cues inhibited cell function. Critically, in combination, topography modulated the effects of chemical environment.
methods
Cell Proliferation
Cell Culture Techniques
Cells
chemistry
Tissue Engineering
Surface Properties
chemistry
methods
metabolism
Humans
metabolism
Cultured
Bone Marrow Cells
Nanostructures
Materials Testing
cytology
Cell Survival
Fibroblasts
cytology
Cell Adhesion
metabolism
physiology
Biocompatible Materials
chemistry
ultrastructure
Substrate Specificity
Proteins
Author
C C Berry
A S G Curtis
R O C Oreffo
Hossein Agheli
Chalmers, Applied Physics, Biological Physics
D S Sutherland
IEEE Transactions on Nanobioscience
1536-1241 (ISSN)
Vol. 6 3 201-9PubMed
17926777