Turn-On, Fluorescent Nuclear Stains with Live Cell Compatibility
Journal article, 2013

DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.


D. R. G. Pitter

University of Miami

Jens Wigenius

Chalmers, Chemical and Biological Engineering, Physical Chemistry

A. S. Brown

University of Miami

J. D. Baker

University of Miami

Fredrik Westerlund

Chalmers, Chemical and Biological Engineering, Physical Chemistry

J. Wilson

University of Miami

Organic Letters

1523-7060 (ISSN) 1523-7052 (eISSN)

Vol. 15 6 1330-1333

Areas of Advance

Nanoscience and Nanotechnology (2010-2017)

Life Science Engineering (2010-2018)

Subject Categories

Chemical Sciences



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