Turn-On, Fluorescent Nuclear Stains with Live Cell Compatibility
Artikel i vetenskaplig tidskrift, 2013
DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.
Författare
D. R. G. Pitter
University of Miami
Jens Wigenius
Chalmers, Kemi- och bioteknik, Fysikalisk kemi
A. S. Brown
University of Miami
J. D. Baker
University of Miami
Fredrik Westerlund
Chalmers, Kemi- och bioteknik, Fysikalisk kemi
J. Wilson
University of Miami
Organic Letters
1523-7060 (ISSN) 1523-7052 (eISSN)
Vol. 15 6 1330-1333Styrkeområden
Nanovetenskap och nanoteknik (SO 2010-2017, EI 2018-)
Livsvetenskaper och teknik (2010-2018)
Ämneskategorier
Kemi
DOI
10.1021/ol400268t