Turn-On, Fluorescent Nuclear Stains with Live Cell Compatibility
Artikel i vetenskaplig tidskrift, 2013

DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.

Författare

D. R. G. Pitter

University of Miami

Jens Wigenius

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

A. S. Brown

University of Miami

J. D. Baker

University of Miami

Fredrik Westerlund

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

J. Wilson

University of Miami

Organic Letters

1523-7060 (ISSN) 1523-7052 (eISSN)

Vol. 15 6 1330-1333

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik

Ämneskategorier

Kemi

DOI

10.1021/ol400268t

Mer information

Skapat

2017-10-08