Lipases efficiently stearate and cutinases acetylate the surface of arabinoxylan films
Journal article, 2013
This is the first report on successful enzyme catalyzed surface esterification of hemicellulose films. Enzyme catalyzed surface acetylation with vinyl acetate and stearation with vinyl stearate were studied on rye arabinoxylan (AX) films. Different surface analytical techniques (FT-IR, TOF-SIMS, ESCA, CA) show that lipases from Mucor javanicus, Rhizopus oryzae and Candida rugosa successfully surface stearate AX films and that a cutinase from Fusarium solani pisi surface acetylates these films. The specificities of cutinase and lipases were also compared, and higher activity was observed for lipases utilizing long alkyl chain substrates while higher activity was observed for cutinase utilizing shorter alkyl chain substrates. The contact angle analysis showed films with increased initial hydrophobicity on the surfaces.
Film
Enzymatic modification
Arabinoxylan
Hemicellulose
Surface esterification
Author
Agnes Stépán
Chalmers, Chemical and Biological Engineering, Polymer Technology
George E Anasontzis
Chalmers, Chemical and Biological Engineering, Industrial biotechnology
T. Matama
University of Minho
A. Cavaco-Paulo
University of Minho
Lisbeth Olsson
Chalmers, Chemical and Biological Engineering, Industrial biotechnology
Wallenberg Wood Science Center (WWSC)
Paul Gatenholm
Chalmers, Chemical and Biological Engineering, Polymer Technology
Wallenberg Wood Science Center (WWSC)
Journal of Biotechnology
0168-1656 (ISSN) 18734863 (eISSN)
Vol. 167 1 16-23Subject Categories
Industrial Biotechnology
DOI
10.1016/j.jbiotec.2013.06.004