Lipases efficiently stearate and cutinases acetylate the surface of arabinoxylan films
Journal article, 2013

This is the first report on successful enzyme catalyzed surface esterification of hemicellulose films. Enzyme catalyzed surface acetylation with vinyl acetate and stearation with vinyl stearate were studied on rye arabinoxylan (AX) films. Different surface analytical techniques (FT-IR, TOF-SIMS, ESCA, CA) show that lipases from Mucor javanicus, Rhizopus oryzae and Candida rugosa successfully surface stearate AX films and that a cutinase from Fusarium solani pisi surface acetylates these films. The specificities of cutinase and lipases were also compared, and higher activity was observed for lipases utilizing long alkyl chain substrates while higher activity was observed for cutinase utilizing shorter alkyl chain substrates. The contact angle analysis showed films with increased initial hydrophobicity on the surfaces.

Film

Enzymatic modification

Arabinoxylan

Hemicellulose

Surface esterification

Author

Agnes Stépán

Chalmers, Chemical and Biological Engineering, Polymer Technology

George E Anasontzis

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

T. Matama

University of Minho

A. Cavaco-Paulo

University of Minho

Lisbeth Olsson

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Wallenberg Wood Science Center (WWSC)

Paul Gatenholm

Chalmers, Chemical and Biological Engineering, Polymer Technology

Wallenberg Wood Science Center (WWSC)

Journal of Biotechnology

0168-1656 (ISSN)

Vol. 167 1 16-23

Subject Categories

Industrial Biotechnology

DOI

10.1016/j.jbiotec.2013.06.004

More information

Latest update

8/24/2018