Lipases efficiently stearate and cutinases acetylate the surface of arabinoxylan films
Artikel i vetenskaplig tidskrift, 2013

This is the first report on successful enzyme catalyzed surface esterification of hemicellulose films. Enzyme catalyzed surface acetylation with vinyl acetate and stearation with vinyl stearate were studied on rye arabinoxylan (AX) films. Different surface analytical techniques (FT-IR, TOF-SIMS, ESCA, CA) show that lipases from Mucor javanicus, Rhizopus oryzae and Candida rugosa successfully surface stearate AX films and that a cutinase from Fusarium solani pisi surface acetylates these films. The specificities of cutinase and lipases were also compared, and higher activity was observed for lipases utilizing long alkyl chain substrates while higher activity was observed for cutinase utilizing shorter alkyl chain substrates. The contact angle analysis showed films with increased initial hydrophobicity on the surfaces.

Film

Enzymatic modification

Arabinoxylan

Hemicellulose

Surface esterification

Författare

Agnes Stépán

Chalmers, Kemi- och bioteknik, Polymerteknologi

George E Anasontzis

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

T. Matama

Universidade do Minho

A. Cavaco-Paulo

Universidade do Minho

Lisbeth Olsson

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Wallenberg Wood Science Center (WWSC)

Paul Gatenholm

Chalmers, Kemi- och bioteknik, Polymerteknologi

Wallenberg Wood Science Center (WWSC)

Journal of Biotechnology

0168-1656 (ISSN)

Vol. 167 1 16-23

Ämneskategorier

Industriell bioteknik

DOI

10.1016/j.jbiotec.2013.06.004