In situ laccase treatment enhances the fermentability of steam-exploded wheat straw in SSCF processes at high dry matter consistencies
Journal article, 2013

This work evaluates the in situ detoxification of inhibitory lignocellulosic broths by laccases to facilitate their fermentation by the xylose-consuming Saccharomyces cerevisiae F12. Treatment of wheat straw slurries with laccases prior to SSCF processes decreased the total phenolic content by 50-80%, reducing the lag phase and increasing the cell viability. After laccase treatment, a negative impact on enzymatic hydrolysis was observed. This effect, together with the low enzymatic hydrolysis yields when increasing consistency, resulted in a decrease in final ethanol yields. Furthermore, when using high substrate loading (20% DM (w/v)), high concentration of inhibitors prevailed in broths and the absence of an extra nitrogen source led to a total cell growth inhibition within the first 24 h in non-treated samples. This inhibition of growth at 20% DM (w/v) was overcome by laccase treatment with no addition of nitrogen, allowing S. cerevisiae F12 to produce more than 22 g/L of ethanol.

S. cerevisiae F12

Lignocellulose

Bioethanol

In situ laccase

Simultaneous saccharification and co-fermentation

Author

David Moreno

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Elia Tomas-Pejo

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

D. Ibarra

Centro de Investigaciones Energeticas, Medioambientales y Tecnologicas (Ciemat)

Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria

M. Ballesteros

Centro de Investigaciones Energeticas, Medioambientales y Tecnologicas (Ciemat)

Lisbeth Olsson

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Bioresource Technology

0960-8524 (ISSN)

Vol. 143 337-343

Driving Forces

Sustainable development

Subject Categories

Industrial Biotechnology

Areas of Advance

Energy

Life Science Engineering (2010-2018)

DOI

10.1016/j.biortech.2013.06.011

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Latest update

9/6/2018 1