In situ laccase treatment enhances the fermentability of steam-exploded wheat straw in SSCF processes at high dry matter consistencies
Artikel i vetenskaplig tidskrift, 2013

This work evaluates the in situ detoxification of inhibitory lignocellulosic broths by laccases to facilitate their fermentation by the xylose-consuming Saccharomyces cerevisiae F12. Treatment of wheat straw slurries with laccases prior to SSCF processes decreased the total phenolic content by 50-80%, reducing the lag phase and increasing the cell viability. After laccase treatment, a negative impact on enzymatic hydrolysis was observed. This effect, together with the low enzymatic hydrolysis yields when increasing consistency, resulted in a decrease in final ethanol yields. Furthermore, when using high substrate loading (20% DM (w/v)), high concentration of inhibitors prevailed in broths and the absence of an extra nitrogen source led to a total cell growth inhibition within the first 24 h in non-treated samples. This inhibition of growth at 20% DM (w/v) was overcome by laccase treatment with no addition of nitrogen, allowing S. cerevisiae F12 to produce more than 22 g/L of ethanol.

Bioethanol

Simultaneous saccharification and co-fermentation

S. cerevisiae F12

In situ laccase

Lignocellulose

Författare

David Moreno

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Elia Tomas-Pejo

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

D. Ibarra

Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria

Centro de Investigaciones Energeticas, Medioambientales y Tecnologicas

M. Ballesteros

Centro de Investigaciones Energeticas, Medioambientales y Tecnologicas

Lisbeth Olsson

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Bioresource Technology

0960-8524 (ISSN)

Vol. 143 337-343

Drivkrafter

Hållbar utveckling

Ämneskategorier

Industriell bioteknik

Styrkeområden

Energi

Livsvetenskaper och teknik

DOI

10.1016/j.biortech.2013.06.011