Differences in gene expression and cytokine levels between newly diagnosed and chronic pediatric ITP.
Journal article, 2013
Immune thrombocytopenia (ITP) is an autoimmune disease where platelets are destroyed prematurely. In the majority of children the disease resolves, but in some it becomes chronic. To investigate whether these 2 phases of the disease are molecularly similar or separate entities we performed DNA microarray analysis (GEO accession number: GSE46922) of T-cells from newly diagnosed children and children with chronic ITP. We found complete separation of the gene expression profiles between the 2 phases of the disease. Furthermore, the gene expression levels of several cytokines differed between the 2 phases of the disease. This was also reflected in plasma with increased levels of interleukin (IL)-16 and TNF-related weak inducer of apoptosis and lower levels of IL-4 in newly diagnosed compared with chronic ITP. Thus, our data indicate that chronic ITP in childhood is a separate disease entity, dissimilar in many aspects to the newly diagnosed phase.
Author
Margareta Jernås
University of Gothenburg
Yu Hou
Shandong University
Frida Strömberg Célind
The Queen Silvia’s Hospital for Pediatric and Adolescents
Linlin Shao
Qilu Hospital of Shandong University
Intawat Nookaew
Chalmers, Chemical and Biological Engineering, Life Sciences
Qian Wang
Qilu Hospital of Shandong University
Xiuli Ju
Qilu Hospital of Shandong University
Karin Mellgren
The Queen Silvia’s Hospital for Pediatric and Adolescents
Hans Wadenvik
University of Gothenburg
Ming Hou
Qilu Hospital of Shandong University
Ministry of Health of People's Republic of China
Bob Olsson
University of Gothenburg
Blood
0006-4971 (ISSN) 1528-0020 (eISSN)
Vol. 122 10 1789-92Subject Categories
Pediatrics
DOI
10.1182/blood-2013-05-502807
PubMed
23869085