Parvovirus B19 VLP recognizes globoside in supported lipid bilayers
Journal article, 2014

Studies have suggested that the glycosphingolipid globoside (Gb4Cer) is a receptor for human parvovirus B19. Virus-like particles bind to Gb4Cer on thin-layer chromatograms, but a direct interaction between the virus and lipid membrane-associated Gb4Cer has been debated. Here, we characterized the binding of parvovirus B19 VP1/VP2 virus-like particles to glycosphingolipids (i) on thin-layer chromatograms (TLCs) and (ii) incorporated into supported lipid bilayers (SLBs) acting as cell-membrane mimics. The binding specificities of parvovirus B19 determined in the two systems were in good agreement; the VLP recognized both Gb4Cer and the Forssman glycosphingolipid on TLCs and in SLBs compatible with the role of Gb4Cer as a receptor for this virus.

Glycosphingolipids

Carbohydrate

Glycobiology

Parvovirus B19 virus-like particles

Glycan

Author

Waqas Nasir

University of Gothenburg

Jonas Nilsson

University of Gothenburg

Sigvard Olofsson

University of Gothenburg

Marta Bally

Chalmers, Applied Physics, Biological Physics

Gustaf E Rydell

University of Gothenburg

Virology

0042-6822 (ISSN) 1089-862X (eISSN)

Vol. 456 1 364-369

Subject Categories

Biophysics

DOI

10.1016/j.virol.2014.04.004

More information

Created

10/7/2017