Parvovirus B19 VLP recognizes globoside in supported lipid bilayers
Artikel i vetenskaplig tidskrift, 2014

Studies have suggested that the glycosphingolipid globoside (Gb4Cer) is a receptor for human parvovirus B19. Virus-like particles bind to Gb4Cer on thin-layer chromatograms, but a direct interaction between the virus and lipid membrane-associated Gb4Cer has been debated. Here, we characterized the binding of parvovirus B19 VP1/VP2 virus-like particles to glycosphingolipids (i) on thin-layer chromatograms (TLCs) and (ii) incorporated into supported lipid bilayers (SLBs) acting as cell-membrane mimics. The binding specificities of parvovirus B19 determined in the two systems were in good agreement; the VLP recognized both Gb4Cer and the Forssman glycosphingolipid on TLCs and in SLBs compatible with the role of Gb4Cer as a receptor for this virus.

Glycosphingolipids

Carbohydrate

Glycobiology

Parvovirus B19 virus-like particles

Glycan

Författare

Waqas Nasir

Göteborgs universitet

Jonas Nilsson

Göteborgs universitet

Sigvard Olofsson

Göteborgs universitet

Marta Bally

Chalmers, Teknisk fysik, Biologisk fysik

Gustaf E Rydell

Göteborgs universitet

Virology

0042-6822 (ISSN) 1089-862X (eISSN)

Vol. 456 1 364-369

Ämneskategorier

Biofysik

DOI

10.1016/j.virol.2014.04.004

Mer information

Skapat

2017-10-07