Gcn4p and the Crabtree effect of yeast: drawing the causal model of the Crabtree effect in Saccharomyces cerevisiae and explaining evolutionary trade-offs of adaptation to galactose through systems biology
Journal article, 2014

By performing an integrated comparative analysis on the physiology and transcriptome of four different S.cerevisiae strains growing on galactose and glucose, it was inferred that the transcription factors Bas1p, Pho2p, and Gcn4p play a central role in the regulatory events causing the Crabtree effect in S.cerevisiae. The analysis also revealed that a point mutation in the RAS2 observed in a galactose-adapted strain causes a lower Crabtree effect and growth rate on glucose by decreasing the activity of Gcn4p while at the same time is at the origin of higher growth rate on galactose due to a lower activity of the transcriptional repressor Sok2p. The role of Gcn4p on the trade-off effect observed on glucose was confirmed experimentally. This was done by showing that the point mutation in RAS2 does not result in a lower growth rate on glucose if it is introduced in a GCN4-negative background.

REGULATOR

random sampling

GLUCOSE REPRESSION

TRANSCRIPTION FACTORS

Bas1

METABOLISM

Gcn4

STRAINS

NETWORKS

PATHWAY

flux analysis

Crabtree effect

Author

Jose Luis Martinez Ruiz

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Sergio Velasco

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Kuk-ki Hong

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

FEMS Yeast Research

1567-1356 (ISSN) 1567-1364 (eISSN)

Vol. 14 4 654-662

Subject Categories

Biological Systematics

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1111/1567-1364.12153

More information

Created

10/7/2017