What do photons do to fluorescently stained DNA in confinement?
Paper in proceedings, 2014

We have studied a selection of factors influencing the damage of DNA in nanochannels during fluorescence imaging. For cutting and nicking of DNA we show that the DNA is shortened during imaging. To avoid photodamage over the course of several hours of a typical experiment, we demonstrate the importance of an oxygen free gas to propel the buffer solution through the device. Finally, by varying the size of the channels, we show indications that higher DNA concentrations lead to higher rates of photodamage necessitating a balance between needs for highly stretched DNA and needs for long measurement times.

Confinement

Photobleaching

Intercalating dye

Nano-channels

DNA

Author

J. P. Beech

Lena Nyberg

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Joachim Fritzsche

Chalmers, Applied Physics, Chemical Physics

Fredrik Westerlund

Chalmers, Chemical and Biological Engineering, Physical Chemistry

J. O. Tegenfeldt

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013

Vol. 1 5-7

Subject Categories

Physical Chemistry

Nano Technology

ISBN

978-163266624-6

More information

Created

10/7/2017