Re-assessment of YAP1 and MCR1 contributions to inhibitor tolerance in robust engineered Saccharomyces cerevisiae fermenting undetoxified lignocellulosic hydrolysate
Journal article, 2014

Development of robust yeast strains that can efficiently ferment lignocellulose-based feedstocks is one of the requirements for achieving economically feasible bioethanol production processes. With this goal, several genes have been identified as promising candidates to confer improved tolerance to S. cerevisiae. In most of the cases, however, the evaluation of the genetic modification was performed only in laboratory strains, that is, in strains that are known to be quite sensitive to various types of stresses. In the present study, we evaluated the effects of overexpressing genes encoding the transcription factor (YAP1) and the mitochondrial NADH-cytochrome b5 reductase (MCR1), either alone or in combination, in an already robust and xylose-consuming industrial strain of S. cerevisiae and evaluated the effect during the fermentation of undiluted and undetoxified spruce hydrolysate. Overexpression of either gene resulted in faster hexose catabolism, but no cumulative effect was observed with the simultaneous overexpression. The improved phenotype of MCR1 overexpression appeared to be related, at least in part, to a faster furaldehyde reduction capacity, indicating that this reductase may have a wider substrate range than previously reported. Unexpectedly a decreased xylose fermentation rate was also observed in YAP1 overexpressing strains and possible reasons behind this phenotype are discussed.

Author

Valeria Wallace-Salinas

Lund University

Lorenzo Signori

University of Milano-Bicocca

Ying-Ying Li

Flanders Interuniversity Institute for Biotechnology

Institute of Botany and Microbiology

Magnus Ask

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Maurizio Bettiga

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Danilo Porro

University of Milano-Bicocca

Johan Thevelein

Institute of Botany and Microbiology

Flanders Interuniversity Institute for Biotechnology

Paola Branduardi

University of Milano-Bicocca

Maria Foulquie-Moreno

Flanders Interuniversity Institute for Biotechnology

Institute of Botany and Microbiology

Marie Francoise Gorwa-Grauslund

Lund University

AMB Express

2191-0855 (ISSN)

Vol. 4 1 56- 56

Driving Forces

Sustainable development

Subject Categories

Industrial Biotechnology

Biochemistry and Molecular Biology

Bioenergy

Areas of Advance

Energy

Life Science Engineering (2010-2018)

DOI

10.1186/s13568-014-0056-5

More information

Latest update

3/2/2018 9