Re-assessment of YAP1 and MCR1 contributions to inhibitor tolerance in robust engineered Saccharomyces cerevisiae fermenting undetoxified lignocellulosic hydrolysate
Artikel i vetenskaplig tidskrift, 2014

Development of robust yeast strains that can efficiently ferment lignocellulose-based feedstocks is one of the requirements for achieving economically feasible bioethanol production processes. With this goal, several genes have been identified as promising candidates to confer improved tolerance to S. cerevisiae. In most of the cases, however, the evaluation of the genetic modification was performed only in laboratory strains, that is, in strains that are known to be quite sensitive to various types of stresses. In the present study, we evaluated the effects of overexpressing genes encoding the transcription factor (YAP1) and the mitochondrial NADH-cytochrome b5 reductase (MCR1), either alone or in combination, in an already robust and xylose-consuming industrial strain of S. cerevisiae and evaluated the effect during the fermentation of undiluted and undetoxified spruce hydrolysate. Overexpression of either gene resulted in faster hexose catabolism, but no cumulative effect was observed with the simultaneous overexpression. The improved phenotype of MCR1 overexpression appeared to be related, at least in part, to a faster furaldehyde reduction capacity, indicating that this reductase may have a wider substrate range than previously reported. Unexpectedly a decreased xylose fermentation rate was also observed in YAP1 overexpressing strains and possible reasons behind this phenotype are discussed.

Författare

Valeria Wallace-Salinas

Lunds universitet

Lorenzo Signori

Universita' degli Studi di Milano-Bicocca

Ying-Ying Li

Flanders Interuniversity Institute for Biotechnology

Institute of Botany and Microbiology

Magnus Ask

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Maurizio Bettiga

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Danilo Porro

Universita' degli Studi di Milano-Bicocca

Johan Thevelein

Institute of Botany and Microbiology

Flanders Interuniversity Institute for Biotechnology

Paola Branduardi

Universita' degli Studi di Milano-Bicocca

Maria Foulquie-Moreno

Flanders Interuniversity Institute for Biotechnology

Institute of Botany and Microbiology

Marie Francoise Gorwa-Grauslund

Lunds universitet

AMB Express

2191-0855 (ISSN)

Vol. 4 1 56- 56

Drivkrafter

Hållbar utveckling

Ämneskategorier

Industriell bioteknik

Biokemi och molekylärbiologi

Bioenergi

Styrkeområden

Energi

Livsvetenskaper och teknik

DOI

10.1186/s13568-014-0056-5