Process optimization of multi-feed SSCF
Conference poster, 2014

Economical production of bio-ethanol from lignocellulosic materials requires an efficient and robust process which enables high-solid fermentation of pretreated lignocellulose to achieve high ethanol fermentation performance. In this work, we design and optimize a high-solid fed-batch simultaneous saccharification and co-fermentation (SSCF) process with a feed of substrate, enzyme and yeast cell for efficient production of ethanol from pretreated wheat straw in both lab and pilot scale. The yeast is prepared by pre-cultivation and adaptation in a semi-continuous cultivation in liquid hydrolysate medium in order to achieve high fermentation capacity. The feeding profiles in both pre-cultivation and SSCF steps are optimized based on a previously developed multi-feed SSCF model [1] in order to maintain high activities of both hydrolytic enzyme and yeast cell resulting in highest biomass yield during pre-cultivation and highest ethanol production efficiency during SSCF process. We also demonstrate scale up of fed-batch SSCF process in a 10 m3 pilot-scale bioreactor. The fed-batch SSCF with an optimized feed of substrate, cell and enzymes reaches high ethanol fermentation performance suggesting it to be a promising process for efficient bioconversion of lignocellulosic materials to ethanol. [1] Wang et al. Bioresour. Technol., 2014

Scale-up

Fed-batch SSCF model

Lignocellulosic ethanol

Author

Ruifei Wang

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Pornkamol Unrean

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Lisbeth Olsson

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Carl Johan Franzén

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

10th European Symposium on Biochemical Engineering Sciences and 6th International Forum on Industrial Bioprocesses

Subject Categories

Biochemicals

Industrial Biotechnology

Bioenergy

Areas of Advance

Energy

Life Science Engineering (2010-2018)

More information

Created

10/7/2017