Carl Johan Franzén

Docent at Department of Biology and Biological Engineering, Industrial Biotechnology

Carl Johan Franzén is Associate Professor in Bioreaction Engineering. His research deals with bioprocess development, kinetic modeling and control of microbial metabolism. He uses network and kinetic modeling and physiologically based control of bioprocesses to optimize production of chemicals using yeast cell factories. One key application is the development of bioethanol production from lignocellulosic raw materials. In recent years, he has developed methodology for encapsulation of yeast to improve the process robustness. The physiological consequences of this has been studied by e.g. proteomics.Carl Johan is also an active teacher and Head of Programme for the five-year MScEng Bioengineering program.

Source: chalmers.se

Projects

2013–2018

Upgrading of renewable domestic raw materials to value-added bulk and fine chemicals for a biobased economy: technology development, systems integration and environmental impact assessment (BioBuF)

Lisbeth Olsson Department of Biology and Biological Engineering, Industrial Biotechnology
Anne-Marie Tillman Department of Energy and Environment, Environmental Systems Analysis
Mathias Janssen Department of Energy and Environment, Environmental Systems Analysis
Carl Johan Franzén Department of Biology and Biological Engineering, Industrial Biotechnology
Elin Svensson Department of Energy and Environment, Energy Technology
Simon Harvey Department of Energy and Environment, Energy Technology
Thore Berntsson Department of Energy and Environment, Energy Technology
Christel Cederberg Department of Energy and Environment, Physical Resource Theory
Hans Theliander Department of Chemistry and Chemical Engineering, Forest Products and Chemical Engineering
Nikolaos Xafenias Department of Biology and Biological Engineering, Industrial Biotechnology
Emma Karlsson Department of Biology and Biological Engineering, Industrial Biotechnology
Maurizio Bettiga Department of Biology and Biological Engineering, Industrial Biotechnology
Karin Pettersson Department of Energy and Environment, Energy Technology
Joshua Mayers Department of Biology and Biological Engineering, Industrial Biotechnology
Eva Albers Department of Biology and Biological Engineering, Industrial Biotechnology
Valeria Mapelli Department of Biology and Biological Engineering, Industrial Biotechnology
Christel Kampman Department of Biology and Biological Engineering, Industrial Biotechnology
Stavros Papadokonstantakis Department of Energy and Environment, Energy Technology
Formas
Västra Götalandsregionen, Regionutvecklingssekretariatet

2013–2015

Scale-Up: Multi-Feed SSF with integrated propagation of flocculating xylose-fermenting yeast

Carl Johan Franzén Department of Biology and Biological Engineering, Industrial Biotechnology
Swedish Energy Agency

2016–2019

Bioethanol from spruce and oatshells via High Gravity Multi-Feed SSF

Carl Johan Franzén Department of Biology and Biological Engineering, Industrial Biotechnology
Swedish Energy Agency

2015–2015

Analysis of present status and opportunities for bioprocesses integrated in biorefineries

Carl Johan Franzén Department of Biology and Biological Engineering, Industrial Biotechnology
Swedish Energy Agency

2014–2015

A comparative analysis of water use in lignocellulosic ethanol processes

Carl Johan Franzén Department of Biology and Biological Engineering, Industrial Biotechnology
Swedish Energy Agency

2013–2014

Skogskemi: Hållbara kemikalier och material

Matteo Morandin Department of Energy and Environment, Industrial Energy Systems and Technologies
Carl Johan Franzén Department of Biology and Biological Engineering, Industrial Biotechnology
Processum Biorefinery Initiative AB

There might be more projects where Carl Johan Franzén participates, but you have to be logged in as a Chalmers employee to see them.

Publications

2016

Model-based optimization and scale-up of multi-feed simultaneous saccharification and co-fermentation of steam pre-treated lignocellulose enables high gravity ethanol production.

Ruifei Wang, Pornkamol Unrean, Carl Johan Franzén et al
Biotechnology for biofuels. Vol. 9, p. 88
Scientific journal article - peer reviewed
2016

Inlärning av tröskelbegreppen inom Bioreaktionsteknik - studentdriven utveckling av en webbaserad modul för självstudier

Palmquist Elena, Maria Bohman, Isabella Palmgren et al
KUL, Chalmers, 2016
Conference paper - peer reviewed
2015

Characterization of the respiratory physiology of Lactococcus lactis for starter culture production with improved acidification capacity

Bettina Lorantfy, Carl Johan Franzén, Lisbeth Olsson et al
Microbial Stress: From Molecules to Systems
Conference paper - non peer reviewed
2015

What induces respiration in lactic acid bacteria? Characterization of respiratory metabolism of Lactococcus lactis in bioreactors for production of starter cultures with improved acidification capacity

Bettina Lorantfy, Carl Johan Franzén, Lisbeth Olsson et al
RAFT 11 Recent Advances in Fermentation Technology
Conference poster
2015

Multifeed simultaneous saccharification and fermentation enables high gravity submerged fermentation of lignocellulose.

Carl Johan Franzén, Ruifei Wang, Johan Westman et al
Recent Advances in Fermentation Technology (RAFT 11), Clearwater Beach, Florida, USA, November 8-11, 2015. Oral presentation.
Conference paper - non peer reviewed
2015

A FLO1 variant which yields a NewFlo phenotype

Johan Westman, Jonas Nyman, Rich Manara et al
32nd International Specialized Symposium on Yeasts, Perugia, Italy, September 13-17, 2015
Conference poster
2015

Factors affecting the viability of Saccharomyces cerevisiae in Simultaneous Saccharification and co-Fermentation of pretreated wheat straw to ethanol

Johan Westman, Ruifei Wang, Vera Novy et al
32nd International Specialized Symposium on Yeasts
Conference poster
2015

Scale-up of multi feed fed-batch simultaneous saccharification and co-fermentation of pretreated wheat straw to ethanol

Johan Westman, Ruifei Wang, Pornkamol Unrean et al
37th Symposium on Biotechnology for Fuels and Chemicals
Conference poster
2015

Kinetic modeling-based optimization of multi-feed simultaneous saccharification and co-fermentation of wheat straw for ethanol production

Ruifei Wang, Johan Westman, Pornkamol Unrean et al
37th Symposium on Biotechnology for Fuels and Chemicals
Conference paper - non peer reviewed
2015

Current progress in high cell density yeast bioprocesses for bioethanol production

Johan Westman, Carl Johan Franzén,
Biotechnology Journal. Vol. 10 (8), p. 1185-1195
Scientific journal article - peer reviewed
2015

Dynamic flux balancing elucidates NAD(P)H production as limiting response to furfural inhibition in Saccharomyces cerevisiae

U. Pornkamol, Carl Johan Franzén,
Biotechnology Journal. Vol. 10 (8), p. 1248-1258
Scientific journal article - peer reviewed
2014

Flocculation Causes Inhibitor Tolerance in Saccharomyces cerevisiae for Second-Generation Bioethanol Production

Johan Westman, Valeria Mapelli, Mohammad J. Taherzadeh et al
Applied and Environmental Microbiology. Vol. 80 (22), p. 6908-6918
Scientific journal article - peer reviewed
2014

Improved fermentation of inhibitory lignocellulose hydrolysates by Saccharomyces cerevisiae at high local cell density

Johan Westman, Mohammad J. Taherzadeh, Carl Johan Franzén et al
10th European Symposium on Biochemical Engineering Sciences (10th ESBES) and the 6th International Forum on Industrial Bioprocesses (6th IFIBiop), Lille, France, 7-10 September 2014
Conference paper - non peer reviewed
2014

Improved inhibitor tolerance and simultaneous utilisation of hexoses and pentoses during fermentation of inhibitory lignocellulose hydrolysates by yeast at high local cell density

Johan Westman, Valeria Mapelli, Mohammad J. Taherzadeh et al
Lignobiotech III, Concepción, Chile, 26-29 October 2014
Conference paper - non peer reviewed
2014

Novel process strategies lead towards efficient bioethanol production at high solid loadings

Rakesh Koppram, Charilaos Xiros, Ruifei Wang et al
Bioeconomy development in Sweden
Conference poster
2014

Process optimization of multi-feed SSCF

Ruifei Wang, Pornkamol Unrean, Lisbeth Olsson et al
10th European Symposium on Biochemical Engineering Sciences and 6th International Forum on Industrial Bioprocesses
Conference poster
2014

Kinetic modeling of multi-feed simultaneous saccharification and co-fermentation of pretreated birch to ethanol

Ruifei Wang, Rakesh Koppram, Lisbeth Olsson et al
Bioresource Technology. Vol. 172, p. 303-311
Scientific journal article - peer reviewed
2014

Continuous ethanol production with a membrane bioreactor at high acetic acid concentrations

P. Ylitervo, Carl Johan Franzén, M.J. Taherzadeh et al
Membranes. Vol. 4 (3), p. 372-387
Scientific journal article - peer reviewed
2014

Improved sugar co-utilisation by encapsulation of a recombinant Saccharomyces cerevisiae strain in alginate-chitosan capsules

Johan Westman, Nicklas Bonander, M. J. Taherzadeh et al
Biotechnology for Biofuels. Vol. 7
Scientific journal article - peer reviewed
2013

Systems biology methods and developments for Saccharomyces cerevisiae and other industrial yeasts in relation to the production of fermented food and food ingredients

Valeria Mapelli, Carl Johan Franzén, Lisbeth Olsson et al
Microbial Production of Food Ingredients, Enzymes and Nutraceuticals, p. 42-80
Chapter in monograph, book
2013

High local cell density for efficient 2nd generation bioethanol production by Saccharomyces cerevisiae

Johan Westman, Valeria Mapelli, Mohammad J. Taherzadeh et al
35th Symposium on Biotechnology for Fuels and Chemicals, April 29-May 2, 2013. Portland Oregon, USA
Conference poster
2013

Together we are strong – Yeast flocculation for efficient fermentation of toxic hydrolysates

Johan Westman, Valeria Mapelli, Mohammad J. Taherzadeh et al
5th Conference on Physiology of Yeast and Filamentous Fungi, 4-7 June, 2013, Montpellier, France
Conference poster
2013

Rapid ethanol production by Saccharomyces cerevisiae in a membrane bioreactor

Päivi Ylitervo, Carl Johan Franzén, Mohammad J. Taherzadeh et al
35th Symposium on Biotechnology for Fuels and Chemicals
Conference poster
2013

Impact of Furfural on Rapid Ethanol Production Using a Membrane Bioreactor

Päivi Ylitervo, Carl Johan Franzén, Mohammad J. Taherzadeh et al
Energies. Vol. 6 (3), p. 1604-1617
Scientific journal article - peer reviewed
2012

A novel process configuration of Simultaneous Saccharification and Fermentation for bioethanol production at high solid loadings

Rakesh Koppram, Ruifei Wang, Carl Johan Franzén et al
Advanced Biofuels in a Biorefinery Approach, February 28 - March 1, 2012, Copenhagen, Denmark
Conference poster
2012

A novel process configuration of Simultaneous Saccharification and Fermentation for bioethanol production at high solid loadings

Rakesh Koppram, Ruifei Wang, Carl Johan Franzén et al
34th Symposium on Biotechnology for fuels and chemicals, April 30 – May 3, 2012, New Orleans, LA
Conference poster
2012

Robust polysiloxane-ACA capsules for ethanol production from wood hydrolyzate by yeast

Päivi Ylitervo, Carl Johan Franzén, Mohammad J. Taherzadeh et al
Life Science Engineering Area of Advance Conference, From Human health to Biosustainability – Future challenges for Life Science at Chalmers.
Conference poster
2012

Robust liquid core APTES-alginate-chitosan-alginate capsules for 2nd generation bioethanol production

Päivi Ylitervo, Carl Johan Franzén, Mohammad J. Taherzadeh et al
34th Symposium on Biotechnology for Fuels and Chemicals
Conference poster
2012

Mechanically robust polysiloxane – ACA capsules for prolonged ethanol production

Päivi Ylitervo, Carl Johan Franzén, Mohammad J. Taherzadeh et al
Journal of chemical technology and biotechnology. Vol. 88 (6), p. 1080–1088
Scientific journal article - peer reviewed
2012

Physiological consequences of encapsulating Saccharomyces cerevisiae: A proteomic approach

Johan Westman, Mohammad J. Taherzadeh, Carl Johan Franzén et al
Nordic Yeast Research Community Symposium, August 30-31, Göteborg, Sweden
Conference paper - non peer reviewed
2012

Proteomic analysis of the increased stress tolerance of Saccharomyces cerevisiae encapsulated in liquid core alginate-chitosan capsules

Johan Westman, Mohammad J. Taherzadeh, Carl Johan Franzén et al
PLoS ONE. Vol. 7 (11), p. e49335
Scientific journal article - peer reviewed
2012

Investigations of the inhibitor tolerance of encapsulated Saccharomyces cerevisiae

Johan Westman, Mohammad J. Taherzadeh, Carl Johan Franzén et al
From Human Health to Biosustainability – Future Challenges for Life Science at Chalmers, 19 November 2012
Conference poster
2012

Physiological consequences of encapsulating Saccharomyces cerevisiae: A proteomic approach

Johan Westman, Mohammad J. Taherzadeh, Carl Johan Franzén et al
13th International Congress on Yeasts, 26-30 August, Madison, Wisconsin, USA
Conference poster
2012

Physiological consequenses of high local cell density Saccharomyces cerevisiae cultures

Johan Westman, Mohammad J. Taherzadeh, Carl Johan Franzén et al
Science and Technology Day 2012, Dept Chemical and Biological Engineering, Chalmers, Gothenburg, Sweden
Conference poster
2012

Inhibitor tolerance by high local cell density Saccharomyces cerevisiae cultures

Johan Westman, Mohammad J. Taherzadeh, Carl Johan Franzén et al
34th Symposium on Biotechnology for Fuels and Chemicals, April 30 - May 2, 2012, New Orleans, USA
Conference poster
2012

Effects of encapsulation of microorganisms on product formation during microbial fermentations

Johan Westman, Päivi Ylitervo, Carl Johan Franzén et al
Applied Microbiology and Biotechnology. Vol. 96 (6), p. 1441-1454
Scientific journal article - peer reviewed
2012

Encapsulation-Induced Stress Helps Saccharomyces cerevisiae Resist Convertible Lignocellulose Derived Inhibitors

Johan Westman, R. B. Manikondu, Carl Johan Franzén et al
International Journal of Molecular Sciences. Vol. 13 (9), p. 11881-11894
Scientific journal article - peer reviewed
2012

Inhibitor tolerance and flocculation of a yeast strain suitable for second generation bioethanol production

Johan Westman, M. J. Taherzadeh, Carl Johan Franzén et al
Electronic Journal of Biotechnology. Vol. 15 (3)
Scientific journal article - peer reviewed
2011

THERMOTOLERANCE OF ENCAPSULATED YEAST

Päivi Ylitervo, Mohammad J. Taherzadeh, Carl Johan Franzén et al
Abstract book, Joint poster exhibition at the Dept Chemical and Biological Engineering, Chalmers University of Technology, and Dept Chemistry, University of Gothenburg. April 12, Göteborg, Sweden
Conference poster
2011

Inhibitor tolerance and flocculation: Characterization of a yeast strain suitable for 2nd generation bioethanol production

Johan Westman, Mohammad J. Taherzadeh, Carl Johan Franzén et al
Abstract book, Joint poster exhibition at the Dept Chemical and Biological Engineering, Chalmers University of Technology, and Dept Chemistry, University of Gothenburg. April 12, Göteborg, Sweden
Conference poster
2011

Encapsulated vs. free yeast: A comparative proteomic study

Johan Westman, Mohammad J. Taherzadeh, Carl Johan Franzén et al
International PhD course in Industrial Biotechnology for lignocellulose based processes, October 16-21, Göteborg, Sweden
Conference poster
2011

A novel process configuration of simultaneous saccharification and fermentation for bioethanol production at high solid loadings

Rakesh Koppram, Carl Johan Franzén, Eva Albers et al
ISAF2011, 10-14 October 2011, Verona, Italy
Conference paper - non peer reviewed
2011

Encapsulation of yeast for efficient 2nd generation bioethanol production: Finite element modeling of concentration profiles in encapsulated yeast

Joana Paula Da Costa Pereira, Goncalo Carvalho Esteves, Carl Johan Franzén et al
IV International Conference on Environmental, Industrial and Applied Microbiology, September 14-16, Torremolinos, Spain
Conference poster
2011

Selective suppression of bacterial contaminants by process conditions during lignocellulose based yeast fermentations

Eva Albers, Emma Johansson, Carl Johan Franzén et al
Biotechnology for Biofuels. Vol. 4
Scientific journal article - peer reviewed
2011

Ethanol production at elevated temperatures using encapsulation of yeast

Päivi Ylitervo, Carl Johan Franzén, M. J. Taherzadeh et al
Journal of Biotechnology. Vol. 156 (1), p. 22-29
Scientific journal article - peer reviewed
2010

Improving the stability and mechanical resistance of chitosan/alginate capsules for encapsulation of S. cerevisiae

Päivi Ylitervo, Carl Johan Franzén, Mohammad J. Taherzadeh et al
International Workshop on Wood Biorefinery and Tree Biotechnology, Örnsköldsvik, Sweden
Conference poster
2010

Increasing the thermotolerance of Saccharomyces cerevisiae by encapsulation

Päivi Ylitervo, Carl Johan Franzén, Mohammad J. Taherzadeh et al
XVIII International Conference on Bioencapsulation, Porto, Portugal
Conference poster
2010

Together we are strong! Inhibitor tolerance conferred by good neighbors?

Johan Westman, Farid Talebnia, Carl Johan Franzén et al
Industrial Systems Biology: Sustainable Production of Fuels and Chemicals, Gothenburg, Sweden, August 2010 and XVIII International Conference on Bioencapsulation, Porto, Portugal, October 2010
Conference poster
2010

Phenotypical and Physiological Characterization of a Flocculating Yeast Strain

Johan Westman, Carl Johan Franzén, Mohammad J. Taherzadeh et al
Physiology of Yeast and Filamentous Fungi (PYFF4), Rotterdam, Holland, June 2010 and 2010 International Workshop on Wood Biorefinery and Tree Biotechnology, Örnsköldsvik, Sweden, June 2010
Conference poster
2010

On-line monitoring of fermentation processes in lignocellulose-to-bioalcohol production.

Anna Eliasson Lantz, Krist V. Gernaey, Carl Johan Franzén et al
Bioalcohol production: Biochemical conversion of lignocellulosic biomass, p. 315-339
Chapter in monograph, book
2009

Ethanol production from lignocellulosic materials by encapsulated Saccharomyces cerevisiae

Päivi Ylitervo, Hamidreza Barghi, Carl Johan Franzén et al
Programme Conference of the Swedish Energy Agency Programme: Ethanol from Cellulose
Conference poster
2007

The impact of severe nitrogen limitation and microaerobic conditions on extended continuous cultivations of Saccharomyces cerevisiae with cell recirculation

Tomas Brandberg, Lena Gustafsson, Carl Johan Franzén et al
Enzyme and Microbial Technology. Vol. 40 (4), p. 585-593
Scientific journal article - peer reviewed
2007

Characterization of glucose transport mutants of saccharomyces cerevisiae during a nutritional upshift reveals a correlation between metabolite levels and glycolytic flux

Daniel Bosch, Mikael Johansson, Cecilia Ferndahl et al
Fems Yeast research. Vol. 8, p. 10-25
Scientific journal article - peer reviewed
2007

Continuous fermentation of wheat-supplemented lignocellulose hydrolysate with different types of cell retention

Tomas Brandberg, Keikhosro Karimi, Mohammad J. Taherzadeh et al
Biotech. Bioeng.. Vol. 98 (1), p. 80-90
Scientific journal article - peer reviewed
2007

Conservation laws and identifiability of rate expressions in biochemical models

Milena Anguelova, Gunnar Cedersund, Carl Johan Franzén et al
IET Systems Biology. Vol. 1 (4), p. 230-237
Scientific journal article - peer reviewed
2005

Continuous fermentation of undetoxified dilute acid lignocellulose hydrolysate with Saccharomyces cerevisiae ATCC 96581 using cell recirculation

Tomas Brandberg, Nima Sanandaji, Lena Gustafsson et al
Biotechnology Progress. Vol. 21, p. 1093-1101
Scientific journal article - peer reviewed
2004

The fermentation performance of nine strains of Saccharomyces cerevisiae in batch and fed-batch cultures in dilute-acid wood hydrolysate

Tomas Brandberg, Carl Johan Franzén, Lena Gustafsson et al
J Biosci Bioeng. Vol. 98, p. 122-125
Scientific journal article - peer reviewed
2003

Effects of furfural on the respiratory metabolism of Saccharomyces cerevisiae in glucose-limited chemostats

Ilona Sárvári Horváth, Carl Johan Franzén, Claes Niklasson et al
Applied and Environmental Microbiology. Vol. 69, p. 4076-4086
Scientific journal article - peer reviewed
2003

Carbon starvation can induce energy deprivation and a loss of fermentative capacity in Saccharomyces cerevisiae.

Elisabeth Thomsson, Christer Larsson, Eva Albers et al
Applied and Environmental Microbiology. Vol. 69, p. 3251-3257
Scientific journal article - peer reviewed
2003

Metabolic flux analysis of RQ-controlled microaerobic ethanol production by Saccharomyces cerevisiae

Carl Johan Franzén,
Yeast. Vol. 20, p. 117-132
Scientific journal article - peer reviewed
2002

Metabolic flux analysis of RQ controlled microaerobic ethanol production by Saccharomyces cerevisiae,

Carl Johan Franzén, Claes Niklasson,
IEEC
Conference paper - peer reviewed
2000

Growth and product formation in anaerobic and microaerobic continuous cultures of Saccharomyces cerevisiae under carbon- and nitrogen limitation.

Carl Johan Franzén, Per Alsin, Claes Niklasson et al
Thermo Chimica Acta
Scientific journal article - peer reviewed
2000

Microaerobic glycerol formation in Saccharomyces cerevisiae

Roeland Costenoble, Hadi Valadi, Claes Niklasson et al
Yeast. Vol. 16, p. 1483-1495
Scientific journal article - peer reviewed
1998

Modeling the fluxes of reducing equivalents in microaerobic RQ-controlled continuous cultures of Saccharomyces cerevisiae.

Carl Johan Franzén, Claes Niklasson,
Larsson C, Påhlman I-L, and Gustafsson L (eds.): BioThermoKinetics in the Post Genomic Era, p. 383-387
Conference paper - peer reviewed
1996

Use of the inlet gas composition to control the respiratory quotient in microaerobic bioprocesses.

Carl Johan Franzén, Eva Albers, Claes Niklasson et al
Chemical Engineering Science. Vol. 51, p. 3391-3402
Scientific journal article - peer reviewed
1994

An experimental guide to the relevant aeration rates in microaerobic bioprocesses.

Carl Johan Franzén, Gunnar Lidén, Claes Niklasson et al
Galindo E and Ramírez O (eds): Advances in Bioprocess Engineering. (Kluwer Academic Publishers), p. 255-265
Conference paper - peer reviewed
1994

A new method for studying microaerobic fermentation: II. An experimental investigation of xylose fermentation.

Carl Johan Franzén, Gunnar Lidén, Claes Niklasson et al
Biotechnology and Bioengineering. Vol. 44, p. 429-435
Scientific journal article - peer reviewed
1994

A new method for studying microaerobic fermentation: I. A theoretical analysis of oxygen programmed fermentation.

Gunnar Lidén, Carl Johan Franzén, Claes Niklasson et al
Biotechnology and Bioengineering. Vol. 44, p. 419-427
Scientific journal article - peer reviewed
1994

Experimental Methods for Microaerobic Bioprocesses

Carl Johan Franzén,
Licentiate thesis