Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation
Journal article, 2019

Lignocellulosic biomass is an abundant and sustainable feedstock, which represents a promising raw material for the production of lactic acid via microbial fermentation. However, toxic compounds that affect microbial growth and metabolism are released from the biomass upon thermochemical pre-treatment. So far, susceptibility of bacterial strains to biomass-derived inhibitors still represents a major barrier to lactic acid production from lignocellulose. Detoxification of the pre-treated lignocellulosic material by water washing is commonly performed to alleviate growth inhibition of the production microorganism and achieve higher production rates.

In this study, we assessed the feasibility of replacing the washing step with integrated cellular adaptation during pre-culture of Bacillus coagulans MA-13 prior to simultaneous saccharification and lactic acid fermentation of steam exploded wheat straw. Using a seed culture pre-exposed to 30% hydrolysate led to 50% shorter process time, 50% higher average volumetric and 115% higher average specific productivity than when using cells from a hydrolysate-free seed culture.

Pre-exposure of B. coagulans MA-13 to hydrolysate supports adaptation to the actual production medium. This strategy leads to lower process water requirements and combines cost-effective seed cultivation with physiological pre-adaptation of the production strain, resulting in reduced lactic acid production costs.


Wheat straw

Simultaneous saccharification and fermentation

Bacillus coagulans



Martina Aulitto

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

University of Naples Federico II

Salvatore Fusco

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

University of Naples Federico II

David Nickel

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Simonetta Bartolucci

University of Naples Federico II

Patrizia Contursi

University of Naples Federico II

Carl Johan Franzén

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Biotechnology for Biofuels

1754-6834 (eISSN)

Vol. 12 45

Upgrading of renewable domestic raw materials to value-added bulk and fine chemicals for a biobased economy: technology development, systems integration and environmental impact assessment (BioBuF)

Region Västra Götaland, 2013-11-01 -- 2018-10-31.

Formas, 2013-06-17 -- 2018-12-31.

Bacillus coagulans as microbial cell factory for L-lactic acid production from agricultural and forestry waste

Stiftelsen BLANCEFLOR Boncompagni Ludovisi, född Bildt, -- .

Driving Forces

Sustainable development

Subject Categories


Industrial Biotechnology

Bioprocess Technology

Areas of Advance


Life Science Engineering (2010-2018)



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2/4/2021 9