Encapsulation-Induced Stress Helps Saccharomyces cerevisiae Resist Convertible Lignocellulose Derived Inhibitors
Journal article, 2012

The ability of macroencapsulated Saccharomyces cerevisiae CBS8066 to withstand readily and not readily in situ convertible lignocellulose-derived inhibitors was investigated in anaerobic batch cultivations. It was shown that encapsulation increased the tolerance against readily convertible furan aldehyde inhibitors and to dilute acid spruce hydrolysate, but not to organic acid inhibitors that cannot be metabolized anaerobically. Gene expression analysis showed that the protective effect arising from the encapsulation is evident also on the transcriptome level, as the expression of the stress-related genes YAP1, ATR1 and FLR1 was induced upon encapsulation. The transcript levels were increased due to encapsulation already in the medium without added inhibitors, indicating that the cells sensed low stress level arising from the encapsulation itself. We present a model, where the stress response is induced by nutrient limitation, that this helps the cells to cope with the increased stress added by a toxic medium, and that superficial cells in the capsules degrade convertible inhibitors, alleviating the inhibition for the cells deeper in the capsule.

anaerobic batch fermentation

furfural

acid

pretreatment

microencapsulation

lignocellulosic hydrolysate

conversion

ethanol

carboxylic acids

HMF (5-hydroxymethyl furfural)

glucose

ethanol-production

yeast-cells

hydrolyzate

continuous cultivation

Author

Johan Westman

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

R. B. Manikondu

University of Borås

Carl Johan Franzén

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Mohammad Taherzadeh Esfahani

University of Borås

International Journal of Molecular Sciences

16616596 (ISSN) 14220067 (eISSN)

Vol. 13 9 11881-11894

Driving Forces

Sustainable development

Subject Categories

Industrial Biotechnology

Microbiology

Areas of Advance

Energy

Life Science Engineering (2010-2018)

DOI

10.3390/ijms130911881

More information

Latest update

3/8/2018 9