Encapsulation-Induced Stress Helps Saccharomyces cerevisiae Resist Convertible Lignocellulose Derived Inhibitors
Artikel i vetenskaplig tidskrift, 2012

The ability of macroencapsulated Saccharomyces cerevisiae CBS8066 to withstand readily and not readily in situ convertible lignocellulose-derived inhibitors was investigated in anaerobic batch cultivations. It was shown that encapsulation increased the tolerance against readily convertible furan aldehyde inhibitors and to dilute acid spruce hydrolysate, but not to organic acid inhibitors that cannot be metabolized anaerobically. Gene expression analysis showed that the protective effect arising from the encapsulation is evident also on the transcriptome level, as the expression of the stress-related genes YAP1, ATR1 and FLR1 was induced upon encapsulation. The transcript levels were increased due to encapsulation already in the medium without added inhibitors, indicating that the cells sensed low stress level arising from the encapsulation itself. We present a model, where the stress response is induced by nutrient limitation, that this helps the cells to cope with the increased stress added by a toxic medium, and that superficial cells in the capsules degrade convertible inhibitors, alleviating the inhibition for the cells deeper in the capsule.

anaerobic batch fermentation

furfural

acid

pretreatment

microencapsulation

lignocellulosic hydrolysate

conversion

ethanol

carboxylic acids

HMF (5-hydroxymethyl furfural)

glucose

ethanol-production

yeast-cells

hydrolyzate

continuous cultivation

Författare

Johan Westman

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

R. B. Manikondu

Högskolan i Borås

Carl Johan Franzén

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Mohammad Taherzadeh Esfahani

Högskolan i Borås

International Journal of Molecular Sciences

16616596 (ISSN) 14220067 (eISSN)

Vol. 13 9 11881-11894

Drivkrafter

Hållbar utveckling

Ämneskategorier

Industriell bioteknik

Mikrobiologi

Styrkeområden

Energi

Livsvetenskaper och teknik

DOI

10.3390/ijms130911881