Kinetic modeling of multi-feed simultaneous saccharification and co-fermentation of pretreated birch to ethanol
Journal article, 2014

Fed-batch simultaneous saccharification and fermentation (SSF) is a feasible option for bioethanol production from lignocellulosic raw materials at high substrate concentrations. In this work, a segregated kinetic model was developed for simulation of fed-batch simultaneous saccharification and co-fermentation (SSCF) of steam-pretreated birch, using substrate, enzymes and cell feeds. The model takes into account the dynamics of the cellulase–cellulose system and the cell population during SSCF, and the effects of pre-cultivation of yeast cells on fermentation performance. The model was cross-validated against experiments using different feed schemes. It could predict fermentation performance and explain observed differences between measured total yeast cells and dividing cells very well. The reproducibility of the experiments and the cell viability were significantly better in fed-batch than in batch SSCF at 15% and 20% total WIS contents. The model can be used for simulation of fed-batch SSCF and optimization of feed profiles.

S. cerevisiae

2nd generation biofuel

Enzymatic hydrolysis

Segregated cell model

Fed-batch saccharification and co-fermentation

Author

Ruifei Wang

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Rakesh Koppram

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Lisbeth Olsson

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Carl Johan Franzén

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Bioresource Technology

0960-8524 (ISSN)

Vol. 172 303-311

Areas of Advance

Energy

Life Science Engineering (2010-2018)

Subject Categories

Bioenergy

DOI

10.1016/j.biortech.2014.09.028

More information

Created

10/7/2017