Local release of magnesium from mesoporous TiO2 coatings stimulates the pen-implant expression of osteogenic markers and improves osteoconductivity in vivo
Journal article, 2014

Local release of Mg ions from titanium implant surfaces has been shown to enhance implant retention and integration. To clarify the biological events that lead to this positive outcome, threaded implants coated with mesoporous TiO2 thin films were loaded with Mg-ions and placed in the tibia of rabbits for 3 weeks, after surface characterization. Non-loaded mesoporous coated implants were used as controls. Pen-implant gene expression of a set of osteogenic and inflammatory assays was quantified by means of real-time quantitative polymerase chain reaction. The expression of three osteogenic markers (OC, RUNX-2 and IGF-1) was significantly more pronounced in the test specimens, suggesting that the release of Mg ions directly at the implant sites may stimulate an osteogenic environment. Furthermore, bone healing around implants was evaluated on histological slides and by diffraction enhanced imaging (DEI), using synchrotron radiation. The histological analysis demonstrated new bone formation around all implants, without negative responses, with a significant increase in the number of threads filled with new bone for test surfaces. DEI analysis attested the high mineral content of the newly formed bone. Improved surface osteoconductivity and increased expression of genes involved in the bone regeneration were found for magnesium-incorporation of mesoporous TiO2 coatings.


Gene expression

Diffraction-enhanced imaging

Local drug delivery osseointegration



S. Galli

Malmö university

Y. Naito

Tokushima University Hospital

Johan Karlsson

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

Wenxiao He

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

I. Miyamoto

Kyushu University

Y. Xue

University of Bergen

Martin Andersson

Chalmers, Chemical and Biological Engineering, Applied Surface Chemistry

K. Mustafa

University of Bergen

Ann Wennerberg

Malmö university

Ryo Jimbo

Malmö university

Nagasaki University

Acta Biomaterialia

1742-7061 (ISSN)

Vol. 10 12 5193-5201

Subject Categories

Medical Materials



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