Local release of magnesium from mesoporous TiO2 coatings stimulates the pen-implant expression of osteogenic markers and improves osteoconductivity in vivo
Artikel i vetenskaplig tidskrift, 2014

Local release of Mg ions from titanium implant surfaces has been shown to enhance implant retention and integration. To clarify the biological events that lead to this positive outcome, threaded implants coated with mesoporous TiO2 thin films were loaded with Mg-ions and placed in the tibia of rabbits for 3 weeks, after surface characterization. Non-loaded mesoporous coated implants were used as controls. Pen-implant gene expression of a set of osteogenic and inflammatory assays was quantified by means of real-time quantitative polymerase chain reaction. The expression of three osteogenic markers (OC, RUNX-2 and IGF-1) was significantly more pronounced in the test specimens, suggesting that the release of Mg ions directly at the implant sites may stimulate an osteogenic environment. Furthermore, bone healing around implants was evaluated on histological slides and by diffraction enhanced imaging (DEI), using synchrotron radiation. The histological analysis demonstrated new bone formation around all implants, without negative responses, with a significant increase in the number of threads filled with new bone for test surfaces. DEI analysis attested the high mineral content of the newly formed bone. Improved surface osteoconductivity and increased expression of genes involved in the bone regeneration were found for magnesium-incorporation of mesoporous TiO2 coatings.

Diffraction-enhanced imaging

Gene expression



Local drug delivery osseointegration


S. Galli

Malmö universitet

Y. Naito

Tokushima University Hospital

Johan Karlsson

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

Wenxiao He

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

I. Miyamoto

Kyushu University

Y. Xue

Universitetet i Bergen

Martin Andersson

Chalmers, Kemi- och bioteknik, Teknisk ytkemi

K. Mustafa

Universitetet i Bergen

Ann Wennerberg

Malmö universitet

Ryo Jimbo

Nagasaki University

Malmö universitet

Acta Biomaterialia

1742-7061 (ISSN)

Vol. 10 5193-5201


Medicinska material och protesteknik