Synthetic Biology for Engineering Acetyl Coenzyme A Metabolism in Yeast
Magazine article, 2014

The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels, chemicals, and pharmaceuticals. The use of this cell factory for cost-efficient production of novel fuels and chemicals requires high yields and low by product production. Many industrially interesting chemicals are biosynthesized from acetyl coenzyme A (acetyl-CoA), which serves as a central precursor metabolite in yeast. To ensure high yields in production of these chemicals, it is necessary to engineer the central carbon metabolism so that ethanol production is minimized (or eliminated) and acetyl-CoA can be formed from glucose in high yield. Here the perspective of generating yeast platform strains that have such properties is discussed in the context of a major breakthrough with expression of a functional pyruvate dehydrogenase complex in the cytosol.

Author

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

mBio

2161-2129 (ISSN) 2150-7511 (eISSN)

Vol. 5 6 Art. no. e02153-14

Subject Categories

Microbiology

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1128/mBio.02153-14

More information

Created

10/7/2017