Detection of peptide-based nanoparticles in blood plasma by ELISA
Journal article, 2015
Aims: The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. Materials & Methods: A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. Results: The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl meth-acrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. Conclusions: We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.
Author
G.H. Bode
Maastricht University
K.E. Pickl
Joanneum Research Forschungsgesellschaft mbH
M. Sanchez-Purrà
Chemical Institute of Sarrià
B. Albaiges
Chemical Institute of Sarrià
S. Borrós
Chemical Institute of Sarrià
University Ramon Llull
A.J.G. Pötgens
AplaGen GmbH
C. Schmitz
Ludwig Maximilian University of Munich (LMU)
F.M. Sinner
Joanneum Research Forschungsgesellschaft mbH
Medical University of Graz
M. Losen
Maastricht University
H.W.M. Steinbusch
Maastricht University
H.G. Frank
AplaGen GmbH
Ludwig Maximilian University of Munich (LMU)
P. Martinez-Martinez
Maastricht University
K. Wagemann
Society for Chemical Engineering and Biotechnology
H.A. Klok
Swiss Federal Institute of Technology in Lausanne (EPFL)
R.E. Unger
Johannes Gutenberg University Mainz
T. Pieber
Medical University of Graz
A. Cesàro
University of Trieste
J. F. J. Engbersen
University of Twente
Bengt Herbert Kasemo
Chalmers, Applied Physics, Chemical Physics
M. Moeller
Deutsches Wollforschungsinstitut
R. Korenstein
Tel Aviv University
C. Grandfils
University of Liège
A. Bernkop-Schnuerch
University of Innsbruck
C. Kiparissides
Aristotle University of Thessaloniki
S. Slomkowski
Polish Academy of Sciences
P. Venturini
National Institute of Chemistry
C.M. Paleos
National Center for Scientific Research “Demokritos”
B. Podobnik
Lek Pharmaceuticals
P. Borm
Diagnostics BV
MagnaMedics Diagnostics BV
E.C.A. Van Winden
J. Groll
Deutsches Wollforschungsinstitut
B. Zassler
ThioMatrix GmbH
G. Gregoriadis
Lipoxen Plc
P.K. Nielsen
Novo Nordisk
A. Elouahabi
GlaxoSmithKline
PLoS ONE
1932-6203 (ISSN) 19326203 (eISSN)
Vol. 10 5 Art. no. e0126136- e0126136Subject Categories
Analytical Chemistry
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
DOI
10.1371/journal.pone.0126136