Detection of peptide-based nanoparticles in blood plasma by ELISA
Artikel i vetenskaplig tidskrift, 2015
Aims: The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. Materials & Methods: A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. Results: The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl meth-acrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. Conclusions: We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.
Författare
G.H. Bode
Universiteit Maastricht
K.E. Pickl
Joanneum Research Forschungsgesellschaft mbH
M. Sanchez-Purrà
Institut Quimic de Sarria
B. Albaiges
Institut Quimic de Sarria
S. Borrós
Institut Quimic de Sarria
Universitat Ramon Llull
A.J.G. Pötgens
AplaGen GmbH
C. Schmitz
Ludwig-Maximilians-Universität München (LMU)
F.M. Sinner
Joanneum Research Forschungsgesellschaft mbH
Medizinische Universität Graz
M. Losen
Universiteit Maastricht
H.W.M. Steinbusch
Universiteit Maastricht
H.G. Frank
AplaGen GmbH
Ludwig-Maximilians-Universität München (LMU)
P. Martinez-Martinez
Universiteit Maastricht
K. Wagemann
Society for Chemical Engineering and Biotechnology
H.A. Klok
Ecole Polytechnique Federale de Lausanne (EPFL)
R.E. Unger
Johannes Gutenberg-Universität Mainz
T. Pieber
Medizinische Universität Graz
A. Cesàro
Universita degli Studi di Trieste
J. F. J. Engbersen
Universiteit Twente
Bengt Herbert Kasemo
Chalmers, Teknisk fysik, Kemisk fysik
M. Moeller
Deutsches Wollforschungsinstitut
R. Korenstein
Tel Aviv University
C. Grandfils
Universite de Liège
A. Bernkop-Schnuerch
University of Innsbruck
C. Kiparissides
Aristotelio Panepistimio Thessalonikis
S. Slomkowski
Polish Academy of Sciences
P. Venturini
Kemijski Inštitut
C.M. Paleos
National Center for Scientific Research “Demokritos”
B. Podobnik
Lek Pharmaceuticals
P. Borm
Diagnostics BV
MagnaMedics Diagnostics BV
E.C.A. Van Winden
J. Groll
Deutsches Wollforschungsinstitut
B. Zassler
ThioMatrix GmbH
G. Gregoriadis
Lipoxen Plc
P.K. Nielsen
Novo Nordisk
A. Elouahabi
GlaxoSmithKline
PLoS ONE
1932-6203 (ISSN) 19326203 (eISSN)
Vol. 10 5 Art. no. e0126136- e0126136Ämneskategorier
Analytisk kemi
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
DOI
10.1371/journal.pone.0126136