Detection of peptide-based nanoparticles in blood plasma by ELISA
Artikel i vetenskaplig tidskrift, 2015

Aims: The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. Materials & Methods: A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. Results: The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl meth-acrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. Conclusions: We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.

Författare

G.H. Bode

Universiteit Maastricht

K.E. Pickl

Joanneum Research Forschungsgesellschaft mbH

M. Sanchez-Purrà

Institut Quimic de Sarria

B. Albaiges

Institut Quimic de Sarria

S. Borrós

Universitat Ramon Llull

Institut Quimic de Sarria

A.J.G. Pötgens

AplaGen GmbH

C. Schmitz

Ludwig-Maximilians-Universität München

F.M. Sinner

Medizinische Universität Graz

Joanneum Research Forschungsgesellschaft mbH

M. Losen

Universiteit Maastricht

H.W.M. Steinbusch

Universiteit Maastricht

H.G. Frank

AplaGen GmbH

Ludwig-Maximilians-Universität München

P. Martinez-Martinez

Universiteit Maastricht

K. Wagemann

Society for Chemical Engineering and Biotechnology

H.A. Klok

Ecole Polytechnique Federale De Lausanne

R.E. Unger

Johannes Gutenberg-Universität Mainz

T. Pieber

Medizinische Universität Graz

A. Cesàro

Universita degli Studi di Trieste

J. F. J. Engbersen

Universiteit Twente

Bengt Herbert Kasemo

Chalmers, Teknisk fysik, Kemisk fysik

M. Moeller

Deutsches Wollforschungsinstitut

R. Korenstein

Tel Aviv University

C. Grandfils

Universite de Liège

A. Bernkop-Schnuerch

University of Innsbruck

C. Kiparissides

Aristotle University of Thessaloniki

S. Slomkowski

Institute - Center for Molecular and Macromolecular Studies of the Polish Academy of Sciences

P. Venturini

National Institute of Chemistry Ljubljana

C.M. Paleos

Demokritos National Centre for Scientific Research

B. Podobnik

Lek Pharmaceuticals

P. Borm

MagnaMedics Diagnostics BV

Diagnostics BV

E.C.A. Van Winden

J. Groll

Deutsches Wollforschungsinstitut

B. Zassler

ThioMatrix GmbH

G. Gregoriadis

Lipoxen Plc

P.K. Nielsen

Novo Nordisk

A. Elouahabi

GlaxoSmithKline

PLoS ONE

1932-6203 (ISSN)

Vol. 10 Art. no. e0126136- e0126136

Ämneskategorier

Analytisk kemi

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

DOI

10.1371/journal.pone.0126136