Enhanced growth of neural networks on conductive cellulose-derived nanofibrous scaffolds
Journal article, 2016

The problemof recovery fromneurodegeneration needs new effective solutions. Tissue engineering is viewed as a prospective approach for solving this problemsince it can help to develop healthy neural tissue using supportive scaffolds. This study presents effective and sustainable tissue engineering methods for creating biomaterials from cellulose that can be used either as scaffolds for the growth of neural tissue in vitro or as drug screening models. To reach this goal, nanofibrous electrospun cellulose mats were made conductive via two different procedures: carbonization and addition of multi-walled carbon nanotubes. The resulting scaffolds were much more conductive than untreated cellulose material and were used to support growth and differentiation of SH-SY5Y neuroblastoma cells. The cells were evaluated by scanning electron microscopy and confocal microscopy methods over a period of 15 days at different time points. The results showed that the cellulose-derived conductive scaffolds can provide support for good cell attachment, growth and differentiation. The formation of a neural network occurred within 10 days of differentiation, which is a promising length of time for SH-SY5Y neuroblastoma cells.

Conductive scaffolds

Electrospun cellulose

Cell attachment

Neural network

Author

Volodymyr Kuzmenko

Chalmers, Microtechnology and Nanoscience (MC2), Electronics Material and Systems Laboratory

Theodoros Kalogeropoulos

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Polymer Technology

Johannes Thunberg

Chalmers, Chemistry and Chemical Engineering, Chemistry and Biochemistry, Organic Chemistry

Sara Johannesson

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Polymer Technology

Daniel Hägg

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Polymer Technology

Peter Enoksson

Chalmers, Microtechnology and Nanoscience (MC2), Electronics Material and Systems Laboratory

Wallenberg Wood Science Center (WWSC)

Paul Gatenholm

Wallenberg Wood Science Center (WWSC)

Chalmers, Chemistry and Chemical Engineering, Applied Chemistry, Polymer Technology

Materials Science and Engineering C

0928-4931 (ISSN)

Vol. 58 14-23

Driving Forces

Sustainable development

Areas of Advance

Nanoscience and Nanotechnology (2010-2017)

Production

Life Science Engineering (2010-2018)

Materials Science

Subject Categories

Cell Biology

Neurosciences

Manufacturing, Surface and Joining Technology

Biomaterials Science

Nano Technology

Infrastructure

Nanofabrication Laboratory

DOI

10.1016/j.msec.2015.08.012

PubMed

26478282

More information

Latest update

9/21/2018