ABCE1 is a highly conserved RNA silencing suppressor
Journal article, 2015

ATP-binding cassette sub-family E member 1 (ABCE1) is a highly conserved protein among eukaryotes and archaea. Recent studies have identified ABCE1 as a ribosomerecycling factor important for translation termination in mammalian cells, yeast and also archaea. Here we report another conserved function of ABCE1. We have previously described AtRLI2, the homolog of ABCE1 in the plant Arabidopsis thaliana, as an endogenous suppressor of RNA silencing. In this study we show that this function is conserved: human ABCE1 is able to suppress RNA silencing in Nicotiana benthamiana plants, in mammalian HEK293 cells and in the worm Caenorhabditis elegans. Using co-immunoprecipitation and mass spectrometry, we found a number of potential ABCE1-interacting proteins that might support its function as an endogenous suppressor of RNA interference. The interactor candidates are associated with epigenetic regulation, transcription, RNA processing and mRNA surveillance. In addition, one of the identified proteins is translin, which together with its binding partner TRAX supports RNA interference.

Author

Kairi Kärblane

Competence Centre for Cancer Research

Tallinn University of Technology (TalTech)

Jelena Gerassimenko

Tallinn University of Technology (TalTech)

Competence Centre for Cancer Research

Lenne Nigul

Tallinn University of Technology (TalTech)

Alla Piirsoo

Tallinn University of Technology (TalTech)

Agata Smialowska

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Södertörn University

Karolinska Institutet

Kadri Vinkel

Tallinn University of Technology (TalTech)

Per Kylsten

Södertörn University

Europaskolan

Karl Ekwall

Södertörn University

Karolinska Institutet

Peter Swoboda

Karolinska Institutet

Erkki Truve

Competence Centre for Cancer Research

Tallinn University of Technology (TalTech)

Cecilia Sarmiento

Tallinn University of Technology (TalTech)

Competence Centre for Cancer Research

PLoS ONE

1932-6203 (ISSN) 19326203 (eISSN)

Vol. 10 2 e0116702

Subject Categories

Chemical Sciences

DOI

10.1371/journal.pone.0116702

PubMed

25659154

More information

Latest update

4/5/2022 7