ABCE1 is a highly conserved RNA silencing suppressor
Artikel i vetenskaplig tidskrift, 2015

ATP-binding cassette sub-family E member 1 (ABCE1) is a highly conserved protein among eukaryotes and archaea. Recent studies have identified ABCE1 as a ribosomerecycling factor important for translation termination in mammalian cells, yeast and also archaea. Here we report another conserved function of ABCE1. We have previously described AtRLI2, the homolog of ABCE1 in the plant Arabidopsis thaliana, as an endogenous suppressor of RNA silencing. In this study we show that this function is conserved: human ABCE1 is able to suppress RNA silencing in Nicotiana benthamiana plants, in mammalian HEK293 cells and in the worm Caenorhabditis elegans. Using co-immunoprecipitation and mass spectrometry, we found a number of potential ABCE1-interacting proteins that might support its function as an endogenous suppressor of RNA interference. The interactor candidates are associated with epigenetic regulation, transcription, RNA processing and mRNA surveillance. In addition, one of the identified proteins is translin, which together with its binding partner TRAX supports RNA interference.

Författare

Kairi Kärblane

Competence Centre for Cancer Research

Tallinns tekniska universitet (TalTech)

Jelena Gerassimenko

Tallinns tekniska universitet (TalTech)

Competence Centre for Cancer Research

Lenne Nigul

Tallinns tekniska universitet (TalTech)

Alla Piirsoo

Tallinns tekniska universitet (TalTech)

Agata Smialowska

Chalmers, Biologi och bioteknik, Systembiologi

Södertörns högskola

Karolinska Institutet

Kadri Vinkel

Tallinns tekniska universitet (TalTech)

Per Kylsten

Södertörns högskola

Europaskolan

Karl Ekwall

Södertörns högskola

Karolinska Institutet

Peter Swoboda

Karolinska Institutet

Erkki Truve

Competence Centre for Cancer Research

Tallinns tekniska universitet (TalTech)

Cecilia Sarmiento

Tallinns tekniska universitet (TalTech)

Competence Centre for Cancer Research

PLoS ONE

1932-6203 (ISSN) 19326203 (eISSN)

Vol. 10 2 e0116702

Ämneskategorier

Kemi

DOI

10.1371/journal.pone.0116702

PubMed

25659154

Mer information

Senast uppdaterat

2022-04-05