ABCE1 is a highly conserved RNA silencing suppressor
Artikel i vetenskaplig tidskrift, 2015

ATP-binding cassette sub-family E member 1 (ABCE1) is a highly conserved protein among eukaryotes and archaea. Recent studies have identified ABCE1 as a ribosomerecycling factor important for translation termination in mammalian cells, yeast and also archaea. Here we report another conserved function of ABCE1. We have previously described AtRLI2, the homolog of ABCE1 in the plant Arabidopsis thaliana, as an endogenous suppressor of RNA silencing. In this study we show that this function is conserved: human ABCE1 is able to suppress RNA silencing in Nicotiana benthamiana plants, in mammalian HEK293 cells and in the worm Caenorhabditis elegans. Using co-immunoprecipitation and mass spectrometry, we found a number of potential ABCE1-interacting proteins that might support its function as an endogenous suppressor of RNA interference. The interactor candidates are associated with epigenetic regulation, transcription, RNA processing and mRNA surveillance. In addition, one of the identified proteins is translin, which together with its binding partner TRAX supports RNA interference.

Författare

Kairi Kärblane

Tallinna Tehnikaülikool

Competence Centre for Cancer Research

Jelena Gerassimenko

Tallinna Tehnikaülikool

Competence Centre for Cancer Research

Lenne Nigul

Tallinna Tehnikaülikool

Alla Piirsoo

Tallinna Tehnikaülikool

Agata Smialowska

Chalmers

Karolinska Institutet

Södertörns högskola

Kadri Vinkel

Tallinna Tehnikaülikool

Per Kylsten

Europaskolan

Södertörns högskola

Karl Ekwall

Karolinska Institutet

Södertörns högskola

Peter Swoboda

Karolinska Institutet

Erkki Truve

Tallinna Tehnikaülikool

Competence Centre for Cancer Research

Cecilia Sarmiento

Tallinna Tehnikaülikool

Competence Centre for Cancer Research

PLoS ONE

1932-6203 (ISSN)

Vol. 10 2

Ämneskategorier

Kemi

DOI

10.1371/journal.pone.0116702

PubMed

25659154