Temporally resolved differential proteomic analysis of human ventricular CSF for monitoring traumatic brain injury biomarker candidates.
Journal article, 2009
A shotgun proteomic approach based on nanoflow liquid chromatography (nanoLC) in conjunction with matrix assisted laser desorption/ionization time of flight tandem mass spectrometry (MALDI TOF MS/MS) was utilized to quantitatively analyze the protein content of consecutive ventricular cerebrospinal fluid (CSF) samples of severe traumatic brain injury (TBI) patients on an individual basis. CSF was acquired from the lateral ventricle 1-9 days after the TBI incident by canula drain to investigate temporally resolved protein changes in three patients that required intracranial pressure monitoring during neurointensive care. The samples were subjected to at once tryptic digestion followed by isobaric tag labeling before multiplexed peptide separation and MS analysis. By using this approach, we were able to follow characteristic changes in protein concentrations over time allowing new conclusions to be drawn about ongoing pathological processes during TBI. Certain suggested protein-biomarker candidates for TBI, like acute phase reactants (APRs), fibrinogens (FIB), cystatin C (CC) or more brain specific proteins like glial fibrillary acid protein (GFAP) and neuron-specific enolase (NSE) were found to be significantly up-regulated which is in strong consistence with previously reported results. This methodology appears to be a promising tool for studying candidate biomarkers of neurovascular and traumatic brain injuries in the neurointensive care setting.
Intracranial Hypertension
etiology
Adolescent
Mass
cerebrospinal fluid
surgery
Disease Progression
Adult
Male
Brain Injuries
Biomarkers
Matrix-Assisted Laser Desorption-Ionization
Humans
metabolism
Ventriculostomy
Chromatography
physiopathology
Lateral Ventricles
Prognosis
Predictive Value of Tests
Young Adult
physiology
cerebrospinal fluid
methods
Monitoring
Nerve Tissue Proteins
methods
diagnosis
Time Factors
Cerebrospinal Fluid Proteins
analysis
Proteomics
Up-Regulation
physiopathology
Physiologic
methods
Spectrometry
physiology
analysis
Liquid
diagnosis
cerebrospinal fluid
analysis
Author
Jörg Hanrieder
University of Gothenburg
Magnus Wetterhall
Per Enblad
Lars Hillered
Jonas Bergquist
Journal of Neuroscience Methods
0165-0270 (ISSN) 1872678x (eISSN)
Vol. 177 2 469-78Subject Categories
Anesthesiology and Intensive Care
Neurosciences
Neurology
PubMed
19263575