Temporally resolved differential proteomic analysis of human ventricular CSF for monitoring traumatic brain injury biomarker candidates.
Artikel i vetenskaplig tidskrift, 2009

A shotgun proteomic approach based on nanoflow liquid chromatography (nanoLC) in conjunction with matrix assisted laser desorption/ionization time of flight tandem mass spectrometry (MALDI TOF MS/MS) was utilized to quantitatively analyze the protein content of consecutive ventricular cerebrospinal fluid (CSF) samples of severe traumatic brain injury (TBI) patients on an individual basis. CSF was acquired from the lateral ventricle 1-9 days after the TBI incident by canula drain to investigate temporally resolved protein changes in three patients that required intracranial pressure monitoring during neurointensive care. The samples were subjected to at once tryptic digestion followed by isobaric tag labeling before multiplexed peptide separation and MS analysis. By using this approach, we were able to follow characteristic changes in protein concentrations over time allowing new conclusions to be drawn about ongoing pathological processes during TBI. Certain suggested protein-biomarker candidates for TBI, like acute phase reactants (APRs), fibrinogens (FIB), cystatin C (CC) or more brain specific proteins like glial fibrillary acid protein (GFAP) and neuron-specific enolase (NSE) were found to be significantly up-regulated which is in strong consistence with previously reported results. This methodology appears to be a promising tool for studying candidate biomarkers of neurovascular and traumatic brain injuries in the neurointensive care setting.

Intracranial Hypertension

etiology

Adolescent

Mass

cerebrospinal fluid

surgery

Disease Progression

Adult

Male

Brain Injuries

Biomarkers

Matrix-Assisted Laser Desorption-Ionization

Humans

metabolism

Ventriculostomy

Chromatography

physiopathology

Lateral Ventricles

Prognosis

Predictive Value of Tests

Young Adult

physiology

cerebrospinal fluid

methods

Monitoring

Nerve Tissue Proteins

methods

diagnosis

Time Factors

Cerebrospinal Fluid Proteins

analysis

Proteomics

Up-Regulation

physiopathology

Physiologic

methods

Spectrometry

physiology

analysis

Liquid

diagnosis

cerebrospinal fluid

analysis

Författare

Jörg Hanrieder

Göteborgs universitet

Magnus Wetterhall

Per Enblad

Lars Hillered

Jonas Bergquist

Journal of Neuroscience Methods

0165-0270 (ISSN)

Vol. 177 2 469-78

Ämneskategorier

Anestesi och intensivvård

Neurovetenskaper

Neurologi

PubMed

19263575

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2017-10-10