Fluorescence enhancement from single DNA molecules confined in SiO 2 nanochannels
Paper in proceeding, 2010

We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel, an emission enhancement that is only detected for emitted light polarized parallel to the channel. We explain the enhancement effect as being due to optical phenomena in the channels. The enhancement effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.

Emission enhancement

Fluorescence microscopy

Single molecule

Molecules

Optical phenomena

Single DNA molecules

Quantitative fluorescence microscopy

Emission intensity

Fluorescence enhancement

Enhancement effects

DNA

Nanofluidics

Author

Fredrik Westerlund

Chalmers, Chemical and Biological Engineering, Physical Chemistry

University of Gothenburg

Other publications Research

Fredrik Persson

University of Gothenburg

Other publications Research

A. Kristensen

Technical University of Denmark (DTU)

J. O. Tegenfeldt

University of Gothenburg

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Groningen; Netherlands; 3 October 2010 through 7 October 2010

Vol. 1 578-580
9781618390622 (ISBN)

Subject Categories

Physical Sciences

ISBN

9781618390622

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Latest update

2/28/2018

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