Fluorescence enhancement from single DNA molecules confined in SiO 2 nanochannels
Paper i proceeding, 2010

We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel, an emission enhancement that is only detected for emitted light polarized parallel to the channel. We explain the enhancement effect as being due to optical phenomena in the channels. The enhancement effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.

Emission enhancement

Fluorescence microscopy

Single molecule

Molecules

Optical phenomena

Single DNA molecules

Quantitative fluorescence microscopy

Emission intensity

Fluorescence enhancement

Enhancement effects

DNA

Nanofluidics

Författare

Fredrik Westerlund

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Göteborgs universitet

Fredrik Persson

Göteborgs universitet

A. Kristensen

Danmarks Tekniske Universitet (DTU)

J. O. Tegenfeldt

Göteborgs universitet

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Groningen; Netherlands; 3 October 2010 through 7 October 2010

Vol. 1 578-580

Ämneskategorier

Fysik

ISBN

9781618390622