A glucuronoyl esterase from Acremonium alcalophilum cleaves native lignin-carbohydrate ester bonds
Introductory text in journal, 2016

The Glucuronoyl esterases (GE) have been proposed to target lignin-carbohydrate (LC) ester bonds between lignin moieties and glucuronic acid side groups of xylan, but to date, no direct observations of enzymatic cleavage on native LC ester bonds have been demonstrated. In the present investigation, LCC fractions from spruce and birch were treated with a recombinantly produced GE originating from Acremonium alcalophilum (AaGE1). A combination of size exclusion chromatography and 31P NMR analyses of phosphitylated LCC samples, before and after AaGE1 treatment provided the first evidence for cleavage of the LC ester linkages existing in wood.

Author

Jenny Arnling Bååth

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Wallenberg Wood Science Center (WWSC)

Nicola Giummarella

Royal Institute of Technology (KTH)

Sylvia Klaubauf

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Martin Lawoko

Royal Institute of Technology (KTH)

Lisbeth Olsson

Wallenberg Wood Science Center (WWSC)

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

FEBS Letters

0014-5793 (ISSN)

Vol. 590 16 2611-8

Subject Categories

Biochemistry and Molecular Biology

Analytical Chemistry

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1002/1873-3468.12290

More information

Latest update

5/20/2020