A glucuronoyl esterase from Acremonium alcalophilum cleaves native lignin-carbohydrate ester bonds
Journal article, 2016
The Glucuronoyl esterases (GE) have been proposed to target lignin-carbohydrate (LC) ester bonds between lignin moieties and glucuronic acid side groups of xylan, but to date, no direct observations of enzymatic cleavage on native LC ester bonds have been demonstrated. In the present investigation, LCC fractions from spruce and birch were treated with a recombinantly produced GE originating from Acremonium alcalophilum (AaGE1). A combination of size exclusion chromatography and 31P NMR analyses of phosphitylated LCC samples, before and after AaGE1 treatment provided the first evidence for cleavage of the LC ester linkages existing in wood.
Author
Jenny Arnling Bååth
Wallenberg Wood Science Center (WWSC)
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Nicola Giummarella
Royal Institute of Technology (KTH)
Sylvia Klaubauf
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
Martin Lawoko
Royal Institute of Technology (KTH)
Lisbeth Olsson
Wallenberg Wood Science Center (WWSC)
Chalmers, Biology and Biological Engineering, Industrial Biotechnology
FEBS Letters
0014-5793 (ISSN) 18733468 (eISSN)
Vol. 590 16 2611-8Subject Categories
Biochemistry and Molecular Biology
Analytical Chemistry
Areas of Advance
Life Science Engineering (2010-2018)
DOI
10.1002/1873-3468.12290