A glucuronoyl esterase from Acremonium alcalophilum cleaves native lignin-carbohydrate ester bonds
Artikel i vetenskaplig tidskrift, 2016

The Glucuronoyl esterases (GE) have been proposed to target lignin-carbohydrate (LC) ester bonds between lignin moieties and glucuronic acid side groups of xylan, but to date, no direct observations of enzymatic cleavage on native LC ester bonds have been demonstrated. In the present investigation, LCC fractions from spruce and birch were treated with a recombinantly produced GE originating from Acremonium alcalophilum (AaGE1). A combination of size exclusion chromatography and 31P NMR analyses of phosphitylated LCC samples, before and after AaGE1 treatment provided the first evidence for cleavage of the LC ester linkages existing in wood.

Författare

Jenny Arnling Bååth

Wallenberg Wood Science Center (WWSC)

Chalmers, Biologi och bioteknik, Industriell bioteknik

Nicola Giummarella

Kungliga Tekniska Högskolan (KTH)

Sylvia Klaubauf

Chalmers, Biologi och bioteknik, Industriell bioteknik

Martin Lawoko

Kungliga Tekniska Högskolan (KTH)

Lisbeth Olsson

Chalmers, Biologi och bioteknik, Industriell bioteknik

Wallenberg Wood Science Center (WWSC)

FEBS Letters

0014-5793 (ISSN)

Vol. 590 16 2611-8

Ämneskategorier

Biokemi och molekylärbiologi

Analytisk kemi

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1002/1873-3468.12290

Mer information

Senast uppdaterat

2018-08-24