Membrane engineering of S. cerevisiae targeting sphingolipid metabolism
Journal article, 2017

The sustainable production of fuels and chemicals using microbial cell factories is now well established. However, many microbial production processes are still limited in scale due to inhibition from compounds that are present in the feedstock or are produced during fermentation. Some of these inhibitors interfere with cellular membranes and change the physicochemical properties of the membranes. Another group of molecules is dependent on their permeation rate through the membrane for their inhibition. We have investigated the use of membrane engineering to counteract the negative effects of inhibitors on the microorganism with focus on modulating the abundance of complex sphingolipids in the cell membrane of Saccharomyces cerevisiae. Overexpression of ELO3, involved in fatty acid elongation, and AUR1, which catalyses the formation of complex sphingolipids, had no effect on the membrane lipid profile or on cellular physiology. Deletion of the genes ORM1 and ORM2, encoding negative regulators of sphingolipid biosynthesis, decreased cell viability and considerably reduced phosphatidylinositol and complex sphingolipids. Additionally, combining ELO3 and AUR1 overexpression with orm1/2? improved cell viability and increased fatty acyl chain length compared with only orm1/2?. These findings can be used to further study the sphingolipid metabolism, as well as giving guidance in membrane engineering.

Author

Lina Lindahl

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Aline X S Santos

University of Geneva

Friedrich Miescher Institute for Biomedical Research

Helén Olsson

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Lisbeth Olsson

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Maurizio Bettiga

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Scientific Reports

2045-2322 (ISSN)

Vol. 7 41868- 41868

Driving Forces

Sustainable development

Subject Categories

Biochemistry and Molecular Biology

DOI

10.1038/srep41868

More information

Latest update

6/15/2018