Applications of optical DNA mapping in microbiology
Review article, 2017

Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches. In addition, designing lab-on-a-chip systems based on OM is now feasible and will allow the integrated and automated microbiological analysis of biological fluids. Here, we review the basic technology of OM, detail the current state of the art of the field, and look ahead to possible future developments in OM technology for microbiological applications.

microbiological clinical diagnosis

DNA-based identification

optical

imaging technique

lab-on-a chip

Author

D. Bogas

Catholic University of Portugal

Lena Nyberg

Chalmers, Biology and Biological Engineering, Chemical Biology

R. Pacheco

Catholic University of Portugal

N. F. Azevedo

University of Porto

J. P. Beech

Lund University

M. Gomila

University of the Balearic Islands

J. Lalucat

University of the Balearic Islands

C. M. Manaia

Catholic University of Portugal

O. C. Nunes

University of Porto

J. O. Tegenfeldt

Lund University

Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

BioTechniques

0736-6205 (ISSN)

Vol. 62 6 255-267

Subject Categories

Biological Sciences

DOI

10.2144/000114555

More information

Latest update

11/30/2021