Hydrolytic potential of five fungal supernatants to enhance a commercial enzyme cocktail
Journal article, 2017

To evaluate the potential of enzyme cocktails produced by five filamentous fungi to supplement the industrial cellulase cocktail, Celluclast 1.5L, in order to improve the efficiency of saccharification. The fungi were cultivated on wheat bran and the resulting supernatants were combined with Celluclast in enzymatic hydrolysis experiments to test their ability to hydrolyze wheat bran and five cellulose-rich substrates. The supernatant showing the best performance was that from an Aspergillus niger cellulase mutant. The addition of beta-glucosidase only to the Celluclast cocktail was not as beneficial. Supplementing commercial cocktails with enzymes from carefully selected fungi may result in significantly more efficient saccharification of lignocellulosic materials. Furthermore, such an approach could lead to the identification of novel enzyme activities crucial for saccharification.

Trichoderma reesei

Celluclast 1.5L

Wheat bran

Aspergillus niger




Ausra Peciulyte

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Maria Pisano

Chalmers, Biology and Biological Engineering

R. P. de Vries

Utrecht University

Lisbeth Olsson

Chalmers, Biology and Biological Engineering, Industrial Biotechnology

Wallenberg Wood Science Center (WWSC)

Biotechnology Letters

0141-5492 (ISSN) 1573-6776 (eISSN)

Vol. 39 9 1403-1411

Subject Categories

Industrial Biotechnology



More information

Latest update