Hydrolytic potential of five fungal supernatants to enhance a commercial enzyme cocktail
Artikel i vetenskaplig tidskrift, 2017

To evaluate the potential of enzyme cocktails produced by five filamentous fungi to supplement the industrial cellulase cocktail, Celluclast 1.5L, in order to improve the efficiency of saccharification. The fungi were cultivated on wheat bran and the resulting supernatants were combined with Celluclast in enzymatic hydrolysis experiments to test their ability to hydrolyze wheat bran and five cellulose-rich substrates. The supernatant showing the best performance was that from an Aspergillus niger cellulase mutant. The addition of beta-glucosidase only to the Celluclast cocktail was not as beneficial. Supplementing commercial cocktails with enzymes from carefully selected fungi may result in significantly more efficient saccharification of lignocellulosic materials. Furthermore, such an approach could lead to the identification of novel enzyme activities crucial for saccharification.

Celluclast 1.5L

Aspergillus niger

Wheat bran

Trichoderma reesei

Cellulose

Saccharification

Författare

Ausra Peciulyte

Chalmers, Biologi och bioteknik, Industriell bioteknik

Maria Pisano

Chalmers, Biologi och bioteknik

R. P. de Vries

Utrecht University

Lisbeth Olsson

Chalmers, Biologi och bioteknik, Industriell bioteknik

Wallenberg Wood Science Center (WWSC)

Biotechnology Letters

0141-5492 (ISSN) 1573-6776 (eISSN)

Vol. 39 1403-1411

Ämneskategorier

Industriell bioteknik

DOI

10.1007/s10529-017-2371-9