Fluorescence microscopy of nanochannel-confined DNA
Book chapter, 2018
Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level and both the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments and analyze the data.
DNA
Single molecule
Fluorescence
Nanochannels
Author
Fredrik Westerlund
Chalmers, Biology and Biological Engineering, Chemical Biology
F. Persson
Vanadis Diagnostics
Joachim Fritzsche
Chalmers, Physics, Chemical Physics
J. P. Beech
Lund University
J. O. Tegenfeldt
Lund University
Published in
Methods in Molecular Biology
10643745 (ISSN) 1940-6029 (eISSN)
Vol. 1665 p. 173-198Categorizing
Subject Categories (SSIF 2011)
Chemical Sciences
Identifiers
DOI
10.1007/978-1-4939-7271-5_10