Fluorescence microscopy of nanochannel-confined DNA
Book chapter, 2018

Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level and both the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments and analyze the data.


Single molecule




Fredrik Westerlund

Chalmers, Biology and Biological Engineering, Chemical Biology

F. Persson

Vanadis Diagnostics

Joachim Fritzsche

Chalmers, Physics, Chemical Physics

J. P. Beech

Lund University

J. O. Tegenfeldt

Lund University

Methods in Molecular Biology

10643745 (ISSN) 1940-6029 (eISSN)

Vol. 1665 173-198

Subject Categories

Chemical Sciences



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4/5/2022 6