Fluorescence microscopy of nanochannel-confined DNA
Kapitel i bok, 2018
Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level and both the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments and analyze the data.
DNA
Single molecule
Fluorescence
Nanochannels
Författare
Fredrik Westerlund
Chalmers, Biologi och bioteknik, Kemisk biologi
F. Persson
Vanadis Diagnostics
Joachim Fritzsche
Chalmers, Fysik, Kemisk fysik
J. P. Beech
Lunds universitet
J. O. Tegenfeldt
Lunds universitet
Methods in Molecular Biology
10643745 (ISSN) 1940-6029 (eISSN)
Vol. 1665 173-198Ämneskategorier
Kemi
DOI
10.1007/978-1-4939-7271-5_10