Micropipet Writing Technique for Production of Two-Dimensional Lipid Bilayer Nanotube-Vesicle Networks on Functionalized and Patterned Surfaces
Journal article, 2003

We present a micropipet-assisted writing technique for formation of two-dimensional networks of phospholipid vesicles and nanotubes on functionalized and patterned substrates. The substrates are patterned with vesicle-adhesive circular spots (5-7.5 µm in diameter) consisting of a basal layer of biotin on gold and an apical coating of NeutrAvidin in a sandwich manner. The area surrounding the adhesive spots is coated with a phosphatidylcholine bilayer membrane, preventing protein and liposome adhesion. Networks were formed by aspirating a biotin-functionalized giant unilamellar or multilamellar liposome (5-50 µm in diameter) into a ~3 µm inner diameter borosilicate glass micropipet. By using a pressurized-air microejection system, a portion of the liposome is then ejected back into the solution while forming a first vesicle ~3 µm in diameter. This vesicle is placed on an adhesive spot. When the micropipet is moved, a nanotube connection is formed from the first vesicle and is pulled to the next adhesive spot where a second vesicle is ejected. This procedure can then be repeated until the lipid material is consumed in the pipet. The method allows for formation of networks with a large number of nodes and vertexes with well-defined geometry and surface adhesion, and represents a first step toward very large scale integration of nanotube-vesicle networks in, for example, nanofluidic applications.


Kristin Sott

University of Gothenburg

Chalmers, Department of Chemistry and Bioscience

Mattias Karlsson

Department of Physical Chemistry

Johan Pihl

Chalmers, Department of Chemistry and Bioscience

Johan Hurtig

Department of Physical Chemistry

Tatsiana Lobovkina

Chalmers, Department of Chemistry and Bioscience

Owe Orwar

Department of Physical Chemistry


07437463 (ISSN) 15205827 (eISSN)

Vol. 19 9 3904-3910

Subject Categories

Physical Chemistry

Chemical Sciences



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